UH

U. Hanefeld

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Authored

Electrospray ionization mass spectomotry, circular dichroism and SAXS studies of the (S)-hydroxynitrile lyase from Hevea brasilien.

Enzymatic formation and esterfication of (S)-mandelonitrile.
Journal article (2001) - U. Hanefeld, Adrie J.J. Straathof, J.J. Heijnen

Development of a process model to describe the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase in an aqueous-organic biphasic reactor
A process model for the enzymatic synthesis of (R)-cyanohydrins in an aqueous-organic biphasic-stirred tank reactor was developed. The conversion of benzaldehyde into (R)-mandelonitrile, catalyzed by Prunus amygdalus hydroxynitrile lyase at 5°C and pH 5.5, was chosen as a model s ...

CAL-B Catalyzed enantioselective synthesis of cynohydrins - a facile route to versatile building blocks.
Journal article (2000) - U. Hanefeld, YH Li, R.A. Sheldon, T Maschmeyer

Estimation of kinetic parameters by progress curve analysis for the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase.

Cyclopalladated imine catalysts in Heck arylation: search for the catalytic species.

Synthesis of Prelactone B
Journal article (1999) - U. Hanefeld, AM Hooper, J Staunton

Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: a route to simple statin analogues

Study of the (S)-hydroxynitrile lyase from Hevea brasiliensis: mechanistic implications
Journal article (1999) - U. Hanefeld, Adrie J.J. Straathof, J.J. Heijnen

Enzyme Catalytic Synthesis of Optically Active Cyanohydrins.
Journal article (1999) - Y Li, U. Hanefeld, T Maschmeyer

Enantioselective synthesis of tetrasubstituted d-lactones.
Journal article (1999) - AL Cutter, U. Hanefeld, B Wilkinson, PF Leadlay, J Staunton

Enantiospecific synthesis of analogues of the diketide intermediate of the erythromycin polyketide synthase (PKS).

Enantiospecific synthesis of tetrasubstituted delta-lactones.

Evaluating precursor-directed biosynthesis towards novel erythromycins through in vitro studies on a biomodular polyketide synthase.

Evaluating precursor-directed biosynthesis towards novel erythromycins through in vitro studies on a bimodular polyketide synthase
Background: Modular polyketide synthases (PKSs) catalyse the biosynthesis of complex polyketides using a different set of enzymes for each successive cycle of chain extension. Directed biosynthesis starting from synthetic diketides is a potentially valuable route to novel polyket ...

Enantiospecific synthesis of tetrasubstituted δ-lactones
For investigations concerning the selectivity of polyketide synthases (PKSs) which have been rationally engineered through genetic techniques the synthesis of substituted δ-lactones is of great importance. An enantiospecific route towards eight of the possible sixteen stereoisome ...

The thioesterase of the erythromycin-producing polyketide synthase: influence of acyl chain structure on the mode of release of substrate analogues from the acyl enzyme intermediates.

Engineering of a minimal modular polyketide synthase, and targeted alteration of the stereospecificity of polyketide chain extension.

Hydroxynitrile lyases for industrial enantioselective syntheses.

Rational engineering of complex polyketide biosynthesis - Where do we begin?.