Chiral amines are valuable compounds in the pharmaceutical industry. These valuable compounds have been made via organocatalysis, metallocatalysis but in the last two decades also via biocatalysis. Enzymes are highly selective, making them attractive catalysts to target chiral amine synthesis. The aim of this dissertation was to apply different biocatalytic pathways with oxidoreductases to synthesize chiral amines.@en

Chiral amines are valuable compounds for their use as building blocks for pharmaceutical and fine chemical industries. Previously, chiral amines were made with metals as catalysts, but these are unsustainable and difficult to remove from the product. Organocatalysis is a sustainable alternative, but requires chiral ligands, which are costly. Over the last decade, biocatalytic methods have been developed as well. Our goal was to produce chiral amines with our designed bi-enzymatic cascades, containing an ene reductase and amine dehydrogenase. We produced and purified ene reductases and assayed the asymmetric reduction of our proposed substrate scope, consisting of unsaturated ketones and aldehydes. We also investigated the impact of multiple reaction conditions on these performances that are optimal for amine dehydrogenases. Starting with 10 mM of substrate, we obtained concentrations up to 9.7 mM amine. Also, we obtained 3-methylcyclohexylamine with an enantiomeric and diastereomeric excess up to 99%. Therefore, we conclude that this bi-enzymatic cascade is capable of producing chiral amines with both high enantiomeric and diastereomeric excess. Further research to perform cascades on a larger scale is recommended.