XM
X. Meng
5 records found
1
Novel microbial rhodopsins for optogenetics
Engineering, optimization and application ofmicroscopes, software, screening pipelines, and genetically encoded voltage indicators towards imaging neural dynamics
Optogenetics has revolutionized neuroscience in the last decade. In contrast to traditional electrode-based electrophysiology, optogenetics increases the throughput of targeted neurons by orders of magnitude. Genetically targeted populational neuron activities can thus be monitor
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Plasmonic enhancement of fluorescence has been challenging in in vivo imaging applications. We present a study demonstrating the plasmonic enhancement of fluorescent membrane proteins within their native physiological environment using tailored metallic nanoparticles. This work h
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Genetically encoded voltage indicators, particularly those based on microbial rhodopsins, are gaining traction in neuroscience as fluorescent sensors for imaging voltage dynamics with high-spatiotemporal precision. Here we establish a novel genetically encoded voltage indicator c
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Voltage Imaging with Engineered Proton-Pumping Rhodopsins
Insights from the Proton Transfer Pathway
Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage
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Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illus
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