Fluorescence enhancement of fluorophores in live cells using metallic nanoparticles

Conference paper (2024)

Authors

M. Locarno ImPhys/Brinks group - AS
Q. Dong ImPhys/Brinks group - AS
X. Meng ImPhys/Brinks group - AS
C. Glessi ImPhys/Hagen group - AS
Daan Brinks Erasmus Medical Centre, ImPhys/Brinks group - AS
Research Group
ImPhys/Brinks group (AS) (TU Delft)
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Published Date

2024

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

ImPhys/Imaging Physics

Research Group

ImPhys/Brinks group

Abstract

Plasmonic enhancement of fluorescence has been challenging in in vivo imaging applications. We present a study demonstrating the plasmonic enhancement of fluorescent membrane proteins within their native physiological environment using tailored metallic nanoparticles. This work highlights two schemes to influence the distance between the emitting dipoles and the enhancing nanoparticles, namely the addition of nanoparticles in the buffer solution and the incorporation in the polymer matrix at the bottom of the cells. Incorporating biological structures native to the cellular environment offers opportunities for the optimization of in vivo fluorescence imaging methods and the detection of membrane proteins.

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