A compact microscope for voltage imaging

Journal article (2022)

Authors

X. Meng ImPhys/Microscopy Instrumentation & Techniques - AS
Lex Huismans Student
Teun Huijben ImPhys/Computational Imaging - AS
G.V. Szabó ImPhys/Microscopy Instrumentation & Techniques - AS
R.F.C. van Tol EMSD AS-south Project engineers
Izak De Heer Student
S. Ganapathy ImPhys/Microscopy Instrumentation & Techniques - AS
Daan Brinks Erasmus MC, ImPhys/Microscopy Instrumentation & Techniques - AS
Research Group
ImPhys/Microscopy Instrumentation & Techniques (AS) (TU Delft)
Copyright: © 2022 X. Meng, Lex Huismans, T.A.P.M. Huijben, G.V. Szabó, R.F.C. van Tol, Izak De Heer, S. Ganapathy, D. Brinks
DOI: https://doi.org/10.1088/2040-8986/ac5dd5
More Info
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Published Date

2022

Language

English

Faculty

Applied Sciences

Department

ImPhys/Imaging Physics

Research Group

ImPhys/Microscopy Instrumentation & Techniques

Abstract

Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.