PP
P. Pozzi
27 records found
1
Authored
Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an ampli
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Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose
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The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are t
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By taking multiple input-output measurements, it is shown how to determine the input to an optical system that corrects unknown phase aberrations without interferometric measurements or online iterative optimization within a couple of seconds. It is shown to work in simulations a
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In this Letter, we show that a Shack-Hartmann wavefront sensor can be used for the quantitative measurement of the specimen optical path difference (OPD) in an ordinary incoherent optical microscope, if the spatial coherence of the illumination light in the plane of the specim ...
The use of adaptive lenses instead of deformable mirrors can simplify the implementation of an adaptive optics system. The recently introduced Multi-actuator Adaptive Lens (MAL) can be used in closed loop with a wavefront sensor to correct for time-variant wavefront aberrations.
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We present a new method for fast wavefront sensorless correction of phase aberrations in confocal microscopy, based on a physical model of the distribution of fluorescence light rejected by the pinhole. The method is described, and an experimental confirmation of the method is pr
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The quality of fluorescence microscopy images is often impaired by the presence
of sample induced optical aberrations. Adaptive optical elements such as deformable mirrors or spatial light modulators can be used to correct aberrations. However, previously reported techniques ...
of sample induced optical aberrations. Adaptive optical elements such as deformable mirrors or spatial light modulators can be used to correct aberrations. However, previously reported techniques ...
A methodology for retrieving the unknown object distribution and point-spread functions (PSFs) from a set of images acquired in the presence of temporal phase aberrations is presented in this paper. The method works by finding optimal complimentary linear filters for multi-fra ...
Light-sheet fluorescence microscopy techniques commonly use deconvolution to remove the effect of the illumination beam shape on the image formation, reducing the effects of side lobes and increasing the contrast in three-dimensional imaging. Deconvolution requires knowledge o ...
In this manuscript, we present a lens setup for large defocus and astigmatism correction. A deformable defocus lens and two rotational cylindrical lenses are used to control the defocus and astigmatism. The setup is calibrated using a simple model that allows the calculation of t
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Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recov
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We present a reference-less and time-multiplexing phase retrieval method by making use of the digital micromirror device (DMD). In this method, the DMD functions not only as a flexible binary mask which modulates the optical field, but also as a sampling mask for measuring corres
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Pupil filters, represented by binary phase modulation, have been applied to extend the field of view of a light-sheet fluorescence microscope. Optimization has been used, first numerically to calculate the optimum filter structure and then experimentally, to scale and align the n
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This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through
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We investigate the possibility of interferometric optical field sensor with DMD forming a series of sampling interferometers in the pupil of an optical system. Our preliminary experiment validates the feasibility of our approach for the field reconstruction in the case of coheren
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We have experimentally demonstrated a lensless coherent microscope based on direct registration of the complex optical field by sampling the pupil with a sequence of two-point interferometers formed by a digital micromirror device. Complete registration of the complex amplitude i
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Contributed
For wavefront reconstruction, often the combination of a Shack-Hartmann sensor and a reconstruction method utilizing the Cartesian derivatives of Zernike polynomials (the least-squares method) is used, which is known to introduce cross-talk. In Janssen (2014) a new wavefront reco
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