Pupil filters for extending the field-of-view in light-sheet microscopy

Journal article (2016)

Authors

D. Wilding Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
P. Pozzi Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
O.A. Soloviev Flexible Optical B.V., Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Gleb Vdovin Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering , Flexible Optical B.V.
C.J. Sheppard Italian Institute of Technology
M.H.G. Verhaegen Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Research Group
Team Raf Van de Plas (Mechanical, Maritime and Materials Engineering) (TU Delft)
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Published Date

2016

Language

English

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Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Raf Van de Plas

Abstract

Pupil filters, represented by binary phase modulation, have been applied to extend the field of view of a light-sheet fluorescence microscope. Optimization has been used, first numerically to calculate the optimum filter structure and then experimentally, to scale and align the numerically synthesized filter in the microscope. A significant practical extension of the field of view has been observed, making the reported approach a valuable tool on the path to wide-field light-sheet microscopy.

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