The ecotoxicological safety of the water bodies relies on the reduction of micropollutant emissions from wastewater treatment plants (WWTP). The ecotoxicological safety of the water bodies relies on the reduction of micropollutant emissions from wastewater treatment plants (WWTP). Quantification of micropollutant removal at full-scale WWTP is scarce. To our knowledge, the anaerobic conversion rates determined at conventional activated sludge processes are, so far, scarcely available in the literature for most of the micropollutants. In this research, we quantified the biotransformation rate constants and the removal efficiencies of 16 micropollutants (4,5-methylbenzotriazole, azithromycin, benzotriazole, candesartan, carbamazepine, clarithromycin, diclofenac, gabapentin, hydrochlorothiazide, irbesartan, metoprolol, propranolol, sotalol, sulfamethoxazole, trimethoprim, and venlafaxine), under aerobic, anoxic, and anaerobic redox conditions; using as inoculum wastewater and biomass from a full-scale conventional activated sludge (CAS) system in the Netherlands. Clarithromycin was the compound that exhibited the highest aerobic (76%) and anaerobic (78%) removal efficiencies, while gabapentin showed the highest removal under anoxic conditions (91%). A preference for cometabolic biotransformation of the targeted micropollutants was observed. The highest biotransformation rate constants obtained were: at aerobic conditions clarithromycin with 1.46 L.g_SS⁻¹.d⁻¹; at anoxic conditions, gabapentin with 2.36 L.g_SS⁻¹.d⁻¹; and at anaerobic redox conditions clarithromycin with 1.87 L.g_SS⁻¹.d⁻¹. The obtained results of biotransformation rates will allow further modelling of micropollutant removal in CAS systems, under various redox conditions. These biotransformation rates can be added to extended ASM models to predict effluent concentration and optimize targeted advanced oxidation processes allowing savings in the operational costs and increasing the process viability.

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Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several challenges. In this study, we applied an anaerobic sequencing batch reactor (AnSBR) to treat protein-rich wastewater from a slaughterhouse. We focused on identifying the key factors influencing the removal of chemical oxygen demand (COD) and the settling performance of the sludge. The AnSBR achieved a maximum total COD removal of 90%, a protein degradation efficiency exceeding 80%, and a COD to methane conversion efficiency of over 70% at organic loading rates of up to 6.2 g COD L⁻¹ d⁻¹. We found that the variations in both the organic loading rate within the reactor and the hydraulic retention time in the buffer tank had a significant effect on COD removal. The hydraulic retention time in the buffer tank and the reactor, which determined the ammonification efficiencies and the residual carbohydrate concentrations in the reactor liquid, affected the sludge settleability. Furthermore, the genus Clostridium sensu stricto 1, known as protein- and lipids-degraders, was predominant in the reactor. Statistical analysis showed a significant correlation between the core microbiome and ammonification efficiency, highlighting the importance of protein degradation as the governing process in the treatment. Our results will provide valuable insights to optimise the design and operation of AnSBR for efficient treatment of protein-rich wastewater.

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Bitumen fume condensate (BFC) is a hazardous wastewater generated at asphalt reclamation and production sites. BFC contains a wide variety of potentially toxic organic pollutants that negatively affect anaerobic processes. In this study, we chemically characterized BFC produced at an industrial site and evaluated its degradation under anaerobic conditions. Analyses identified about 900 compounds including acetate, polycyclic aromatic hydrocarbons, phenolic compounds, and metal ions. We estimated the half maximal inhibitory concentrations (IC₅₀) of methanogenesis of 120, 224, and 990 mgCOD·L⁻¹ for three types of anaerobic biomass, which indicated the enrichment and adaptation potentials of methanogenic biomass to the wastewater constituents. We operated an AnMBR (7.0 L, 35 °C) for 188 days with a mixture of BFC, phenol, acetate, and nutrients. The reactor showed a maximum average COD removal efficiency of 87.7 ± 7.0 %, that corresponded to an organic conversion rate of 286 ± 71 mgCOD⁻¹·L⁻¹d⁻¹. The microbial characterization of the reactor's biomass showed the acetoclastic methanogen Methanosaeta as the most abundant microorganism (43 %), whereas the aromatic and phenol degrader Syntrophorhabdus was continuously present with abundances up to 11.5 %. The obtained results offer the possibility for the application of AnMBRs for the treatment of BFC or other petrochemical wastewater.

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Phenol conversion under saline thermophilic anaerobic conditions requires the development and sustenance of a highly specialized microbial community. In the present research, an anaerobic membrane bioreactor (AnMBR) fed with an influent containing 0.5 g·L⁻¹ phenol and 6.5 gNa⁺·L⁻¹ was operated at 55 °C for 300 days. Phenol degradation was limited when phenol was the sole substrate. However, the phenol removal efficiency significantly (p < 0.001) increased to 80 % corresponding to a conversion rate of 29 mgPhenol·gVSS⁻¹d⁻¹ when acetate (0.5 gCOD·L⁻¹) was simultaneously provided. Isotopic analysis using 1–¹³C labeled acetate and measuring ¹³CH₄ revealed that acetate was first oxidized to hydrogen and CO₂, prior to methanogenesis, resulting in an increased abundance of hydrogenotrophic methanogens. It is hypothesized that the latter is of crucial importance for achieving effective anaerobic oxidation of phenol and its metabolites. Remarkably, the phenol conversion rate in the membrane-associated biomass was three times higher than in the suspended biomass. The observed difference in the conversion rate could be explained by the presence of an increased abundance of hydrogenotrophic methanogens in the membrane-associated biomass confirmed by a microbial community analysis of Archaea. Benzoate was measured in the permeate suggesting that phenol degradation occurred via the benzoyl-CoA pathway. The results of the current study suggest that syntrophic acetate oxidation coupled with hydrogenotrophic methanogenesis, which results in the presence of an abundant electron sink, plays a key role in enhancing thermophilic phenol degradation. The obtained insights widen the application of anaerobic digestion to treat saline phenolic-rich wastewater at high temperatures.

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Industrial wastewaters generated in chemical industries are often characterized by extreme conditions, such as the presence of complex, recalcitrant, and toxic aromatic compounds, high temperature, and high salinity. The mentioned conditions predominantly occur when chemical industries reduce process water use or strive for closing water loops. For wastewaters with extreme characteristics, conventional wastewater technologies have limitations. However, granular sludge-based or membrane-assisted anaerobic bio-treatment offers many advantages such as in-reactor augmentation of the required microbial species and long sludge retention times, ensuring high metabolic conversion rates per unit of reactor volume, besides low investment costs and low or negligible energy use. Frequently, auto-immobilization or stable anaerobic sludge granulation cannot be guaranteed under extreme conditions. Thus, the application of anaerobic membrane bioreactor (AnMBR) technology for pre-treating industrial wastewaters could offer an alternative solution with several advantages, such as full retention of specific and slow-growing microbial communities, effluents free of suspended solids, and system compactness. The purpose of this thesis is to investigate the applicability of the AnMBR technology for the treatment of chemical wastewater under extreme conditions by studying the impact of high and fluctuating salinity, and high temperature on the conversion of phenol. Phenol was selected as a model aromatic compound because it is commonly found in chemical wastewaters, while it is also a known inhibitor for the anaerobic conversion process. Moreover, this thesis provides additional understanding of the AnMBR operation, including assessment of membrane resistance to filtration, microbial population dynamics under the different conditions. Three laboratory-scale AnMBRs and one upflow anaerobic sludge blanket (UASB) reactor were used to carry out the experiments at varied salinity, phenol concentration and temperature. Different operating conditions were tested to determine the limitations and robustness of the AnMBR and UASB reactor configurations. The high salinity, expressed as sodium concentration, varied between 6 gNa+.L-1 and 37 g Na+.L-1. Influent phenol concentrations from 0.1 gPh.L-1 up to 5 gPh.L-1 were applied. The AnMBR operational temperature was mainly set to the mesophilic range at 35°C, but experiments were also performed in the hypermesophilic (40-45 ºC) and thermophilic (50-55 ºC) range...@en

Treating petrochemical wastewater is a challenge for conventional anaerobic reactors. One example is coal gasification wastewater that, besides its salinity, is rich in toxic and inhibitory aromatics, such as phenol, cresols, and resorcinol. Studies have shown that phenol and p-cresol share the same degradation intermediates, whereas resorcinol is degraded via another route. This study investigated the simultaneous degradation of p-cresol or resorcinol with phenol under anaerobic saline conditions. Batch experiments with anaerobic phenol-degrading biomass were conducted to assess the feasibility of the degradation of p-cresol and resorcinol. Volumetric uptake rates of 11.4 ± 2.4 mg_p-cresol·L^–1d^–1 and 4.2 ± 1.9 mg_resorcinol·L^–1d^–1 were determined. The effect of p-cresol and resorcinol on the specific methanogenic activity and the cell viability in phenol-degrading and non-adapted biomass was assessed. Half maximal inhibitory concentration (IC₅₀) values of 0.73 g_p-cresol·L^-1 and 3.00 g_resorcinol·L^-1 were estimated for phenol-degrading biomass, whereas IC₅₀ values of 0.60 g_p-cresol·L^-1 and 0.25 g_resorcinol·L^-1 were estimated for the non-adapted biomass. p-Cresol caused a higher decrease in the non-damaged cell counts in comparison to resorcinol. Two anaerobic membrane bioreactors under saline conditions [8 g Na⁺·L^–1] were fed with mixtures of either phenol-p-cresol or phenol-resorcinol. At an influent phenol concentration of 2 g·L^-1, maximum conversion rates of 22 mg_p-cresol·gVSS^-1d^–1 and 16 mg_resorcinol·gVSS^–1d^–1 were found. In both AnMBRs, Syntrophorhabdus sp. and Methanosaeta sp. were the most abundant bacteria and methanogen, respectively. The feasibility of simultaneous conversion of phenolic compounds under saline conditions in AnMBRs opens novel perspectives for the high-rate anaerobic treatment of chemical wastewater.

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High salinity is becoming more common in industrial process water and final effluents, particularly when striving to close water loops. There is limited knowledge on the anaerobic treatment of chemical wastewaters characterized by distinct salinity fluctuations. This study investigates the high and fluctuating salinity effects on the conversion capacity and membrane filtration performance of an anaerobic membrane bioreactor (AnMBR) in treating phenol-containing wastewater. The AnMBR operated for 180 days with sodium concentrations between 8 and 37 gNa^+.L⁻¹. At ≤ 26 gNa^+.L⁻¹, approximately 99% COD and phenol removal efficiencies were achieved. At 37 gNa^+.L⁻¹, phenol and COD removal efficiencies decreased to 86 and 82%, respectively, while the biomass specific methanogenic activity was 0.12 ± 0.05 gCOD-CH₄.gVSS^−1.d⁻¹. Due to large salinity fluctuations, phenol and COD removal efficiencies reduced to ≤ 45% but recovered to ≥ 88%. Compared to phenol conversion, methanogenesis was more severely affected. Calculations showed a maximum in-situ phenol conversion rate of 25.5 mgPh.gVSS^−1.d⁻¹. Concomitantly, biomass integrity was compromised, and the median particle size severely dropped from 65.6 to 4.3 μm, resulting in a transmembrane pressure increase above 400 mbar. Cake layer resistance to filtration contributed to 85% of the total resistance. Nonetheless, all biomass was effectively retained in the AnMBR. A change in salinity ≥ 14 gNa^+.L⁻¹ substantially reduced the microbial richness and diversity. The microbial community was dominated by Bacteria belonging to Clostridiales and Archaea of the orders Methanosarcinales and Methanobacteriales. Our findings demonstrate AnMBRs as suitable techniques for treating chemical process water, with possible subsequent reclamation, characterized by high phenols concentrations and largely fluctuating salinity levels.

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Sludge morphology considerably affects the mechanism underlying microbial anaerobic degradation of phenol. Here, we assessed the phenol degradation rate, specific methanogenic activity, electron transport activity, coenzyme F₄₂₀ concentration, and microbial community structure of five phenol-degrading sludge of varying particle sizes (i.e., < 20, 20–50, 50–100, 100–200, and > 200 μm). The results indicated an increase in phenol degradation rate and microbial community structure that distinctly correlated with an increase in sludge particle size. Although the sludge with the smallest particle size (< 20 μm) showed the lowest phenol degradation rate (9.3 mg COD·gVSS⁻¹ day⁻¹), its methanogenic activity with propionic acid, butyric acid, and H₂/CO₂ as substrates was the best, and the concentration of coenzyme F₄₂₀ was the highest. The small particle size sludge did not contain abundant syntrophic bacteria or hydrogenotrophic methanogens, but contained abundant acetoclastic methanogens. Moreover, the floc sizes of the different sludge varied in important phenol-degrading bacteria and archaea, which may dominate the synergistic mechanism. This study provides a new perspective on the role of sludge floc size on the anaerobic digestion of phenol.

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Closing water loops in chemical industries result in hot and highly saline residual streams, often characterized by high strength and the presence of refractory or toxic compounds. These streams are attractive for anaerobic technologies, provided the chemical compounds are biodegradable. However, under such harsh conditions, effective biomass immobilization is difficult, limiting the use of the commonly applied sludge bed reactors. In this study, we assessed the long-term phenol conversion capacity of a lab-scale anaerobic membrane bioreactor (AnMBR) operated at 55°C, and high salinity (18 gNa^+.L^–1). Over 388 days, bioreactor performance and microbial community dynamics were monitored using specific methanogenic activity (SMA) assays, phenol conversion rate assays, volatile fatty acids permeate characterization and Illumina MiSeq analysis of 16S rRNA gene sequences. Phenol accumulation to concentrations exceeding 600 mgPh^.L^–1 in the reactor significantly reduced methanogenesis at different phases of operation, while applying a phenol volumetric loading rate of 0.12 gPh^.L^–1.d^–1. Stable AnMBR reactor performance could be attained by applying a sludge phenol loading rate of about 20 mgPh^.gVSS^–1.d^–1. In situ maximum phenol conversion rates of 21.3 mgPh^.gVSS^–1.d^–1 were achieved, whereas conversion rates of 32.8 mgPh^.gVSS^–1.d^–1 were assessed in ex situ batch tests at the end of the operation. The absence of caproate as intermediate inferred that the phenol conversion pathway likely occurred via carboxylation to benzoate. Strikingly, the hydrogenotrophic SMA of 0.34 gCOD-CH₄^.gVSS^–1.d^–1 of the AnMBR biomass significantly exceeded the acetotrophic SMA, which only reached 0.15 gCOD-CH₄^.gVSS^–1.d^–1. Our results indicated that during the course of the experiment, acetate conversion gradually changed from acetoclastic methanogenesis to acetate oxidation coupled to hydrogenotrophic methanogenesis. Correspondingly, hydrogenotrophic methanogens of the class Methanomicrobia, together with Synergistia, Thermotogae, and Clostridia classes, dominated the microbial community and were enriched during the three phases of operation, while the aceticlastic Methanosaeta species remarkably decreased. Our findings clearly showed that highly saline phenolic wastewaters could be satisfactorily treated in a thermophilic AnMBR and that the specific phenol conversion capacity was limiting the treatment process. The possibility of efficient chemical wastewater treatment under the challenging studied conditions would represent a major breakthrough for the widespread application of AnMBR technology.

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Phenolic industrial wastewater, such as those from coal gasification, are considered a challenge for conventional anaerobic wastewater treatment systems because of its extreme characteristics such as presence of recalcitrant compounds, high toxicity, and salinity. However, anaerobic membrane bioreactors (AnMBRs) are considered of potential interest since they retain all micro-organism that are required for conversion of the complex organics. In this study, the degradation of phenol as main carbon and energy source (CES) in AnMBRs at high salinity (8.0 g Na⁺⋅L^–1) was evaluated, as well as the effect of acetate and an acetate-butyrate mixture as additional CES on the specific phenol conversion rate and microbial community structure. Three different experiments in two lab-scale (6.5 L) AnMBRs (35°C) were conducted. The first reactor (R1) was fed with phenol as the main CES, the second reactor was fed with phenol and either acetate [2 g COD⋅L^–1], or a 2:1 acetate-butyrate [2 g COD⋅L^–1] mixture as additional CES. Results showed that phenol conversion could not be sustained when phenol was the sole CES. In contrast, when the reactor was fed with acetate or an acetate-butyrate mixture, specific phenol conversion rates of 115 and 210 mgPh⋅gVSS^–1 d^–1, were found, respectively. The syntrophic phenol degrader Syntrophorhabdus sp. and the acetoclastic methanogen Methanosaeta sp. were the dominant bacteria and archaea, respectively, with corresponding relative abundances of up to 63 and 26%. The findings showed that dosage of additional CES allowed the development of a highly active phenol-degrading biomass, potentially improving the treatment of industrial and chemical wastewaters.

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Nutrient pollution has become a global environmental issue. Innovative biological nutrient removal (BNR) processes are needed to overcome the drawbacks of conventional technologies. This study evaluates the potential of a hybrid 5-stage Bardenpho - moving bed biofilm reactor (MBBR) process for organic carbon and nutrient removal from municipal wastewater at different hydraulic retention time (HRT) and nitrate recycle ratio (R). Response surface methodology (RSM) based on a central composite design (CCD) of thirteen experiments was applied to optimize the nitrogen and phosphorus conversion of the treatment system. High removal efficiencies of about 98.20%, 92.54%, 94.70% and 96.50% for total chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP) and ammonium, were achieved, respectively. The best performance was observed at HRT of 2, 4, 6, 2.67 and 1.07 h correspondingly in the anaerobic, first anoxic, first aerobic, second anoxic and second aerobic compartments, resulting in a total HRT of 15.74 h with a nitrate recycle ratio of 2. Biofilm nitrifying activity was four times higher than in suspended biomass. The hybrid 5-stage Bardenpho-MBBR process enhanced biological nutrient removal at comparatively short HRT and low R ratio due to biofilm contribution to the conversion.@en

Anaerobic membrane bioreactors (AnMBRs) offer an attractive option for treating industrial wastewaters under extreme conditions that might hamper granulation, biomass retention and reduce biological activity. This study assesses the long-term performance of an upflow anaerobic sludge blanket reactor (UASB) and an AnMBR treating highly saline phenolic wastewater. Analysis of bioreactor conversion, biomass characteristics and microbial community dynamics under increasing sodium and phenol concentrations is presented. The results demonstrated that compared to the UASB, the AnMBR process exhibited higher stability, likely due to its enhanced biomass retention. The AnMBR retained specialized microorganisms under increasing influent concentrations of phenol up to 5 gPh·L−1 and salinity up to 26 gNa+·L−1. In contrast, when the UASB reached this high influent phenol and high sodium concentration, deflocculation of biomass, apparently due to calcium leaching, was observed leading to a severe conversion capacity loss. Microbial community dynamics showed higher species evenness in the AnMBR compared to the UASB, leading to a higher methanogenic ability to respond to disturbances such as high phenol and sodium concentration increases. These findings highlighted the promising features of AnMBR technology, in widening the application potentials of high-rate anaerobic wastewater treatment and overcoming specific challenges in the treatment of chemical wastewater streams under extreme environmental conditions such as high salinity.@en

Industrial wastewaters are becoming increasingly associated with extreme conditions such as the presence of refractory compounds and high salinity that adversely affect biomass retention or reduce biological activity. Hence, this study evaluated the impact of long-term salinity increase to 20 gNa⁺.L⁻¹ on the bioconversion performance and microbial community composition in anaerobic membrane bioreactors treating phenolic wastewater. Phenol removal efficiency of up to 99.9% was achieved at 14 gNa⁺.L⁻¹. Phenol conversion rates of 5.1 mgPh.gVSS⁻¹.d⁻¹, 4.7 mgPh.gVSS⁻¹.d⁻¹, and 11.7 mgPh.gVSS⁻¹.d⁻¹ were obtained at 16 gNa⁺.L⁻¹,18 gNa⁺.L⁻¹ and 20 gNa⁺.L⁻¹, respectively. The AnMBR's performance was not affected by short-term step-wise salinity fluctuations of 2 gNa⁺.L⁻¹ in the last phase of the experiment. It was also demonstrated in batch tests that the COD removal and methane production rate were higher at a K⁺:Na⁺ ratio of 0.05, indicating the importance of potassium to maintain the methanogenic activity. The salinity increase adversely affected the transmembrane pressure likely due to a particle size decrease from 185 μm at 14 gNa⁺.L⁻¹ to 16 μm at 20 gNa⁺.L⁻¹. Microbial community was dominated by bacteria belonging to the Clostridium genus and archaea by Methanobacterium and Methanosaeta genus. Syntrophic phenol degraders, such as Pelotomaculum genus were found to be increased when the maximum phenol conversion rate was attained at 20 gNa⁺.L⁻¹. Overall, the observed robustness of the AnMBR performance indicated an endured microbial community to salinity changes in the range of the sodium concentrations applied.

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Anammox process is considered as a promising technology for removing total nitrogen from low-strength ammonium and phenol-containing wastewater. However, it is still a challenge for the anammox process to treat high-strength ammonium and phenol-containing wastewater. A completely separated partial nitritation and anammox (CSPN/A) process was developed to remove total nitrogen from high-strength phenol-containing wastewater. About 92% of COD, 100% of phenol, and 82.4% of total nitrogen were successfully removed at a NH₄ ⁺-N concentration of 200 mg L⁻¹ with a phenol/NH₄ ⁺-N mass ratio of 0.5 in the CSPN/A process. Furthermore, a shock loading of 300 mg phenol L⁻¹ with a phenol/NH₄ ⁺-N mass ratio of 1.5 led to a complete failure of partial nitritation, but the performance was rapidly recovered by the increase of NH₄ ⁺-N concentration. Although the activities of ammonium-oxidizing bacteria and anammox bacteria were severely inhibited at a phenol/NH₄ ⁺-N mass ratio of 1.5, the enrichment of efficient phenol degraders in the CSPN stage could strengthen the performance robustness of partial nitritation and anammox process. Therefore, this study presented a new insight on the feasibility of the anammox process for treating high-strength ammonium and phenol-containing wastewater.

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This study examined the temperature susceptibility of a continuous-flow lab-scale anaerobic membrane bioreactor (AnMBR) to temperature shifts from 35 °C to 55 °C and its bioconversion robustness treating synthetic phenolic wastewater at 16 gNa^+.L⁻¹. During the experiment, the mesophilic reactor was subjected to stepwise temperature increases by 5 °C. The phenol conversion rates of the AnMBR decreased from 3.16 at 35 °C to 2.10 mgPh^.gVSS^−1.d⁻¹ at 45 °C, and further decreased to 1.63 mgPh^.gVSS^−1.d⁻¹ at 50 °C. At 55 °C, phenol conversion rate stabilized at 1.53 mgPh^.gVSS^−1.d⁻¹ whereas COD removal efficiency was 38% compared to 95.5% at 45 °C and 99.8% at 35 °C. Interestingly, it was found that the phenol degradation process was less susceptible for the upward temperature shifts than the methanogenic process. The temperature increase implied twenty-one operational taxonomic units from the reactor's microbial community with significant differential abundance between mesophilic and thermophilic operation, and eleven of them are known to be involved in aromatic compounds degradation. Reaching the upper-temperature limits for mesophilic operation was associated with the decrease in microbial abundance of the phyla Firmicutes and Proteobacteria, which are linked to syntrophic phenol degradation. It was also found that the particle size decreased from 89.4 μm at 35 °C to 21.0 μm at 55 °C. The accumulation of small particles and higher content of soluble microbial protein-like substances led to increased transmembrane pressure which negatively affected the filtration performance. Our findings indicated that at high salinity a mesophilic AnMBR can tolerate a temperature up to 45 °C without being limited in the phenol conversion capacity.

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Biomass requires trace metals (TM) for maintaining its growth and activity. This study aimed to determine the effect of TM supplementation and partitioning on the specific methanogenic activity (SMA), with a focus on cobalt and tungsten, during the start-up of two lab-scale Anaerobic Membrane Bioreactors (AnMBRs) treating saline phenolic wastewater. The TM partitioning revealed a strong accumulation of sodium in the biomass matrix and a wash-out of the majority of TM in the reactors, which led to an SMA decrease and a low COD removal of about 30%. The SMA exhibits a maximum at about 6 g Na⁺ L⁻¹ and nearly complete inhibition at 34 g Na⁺ L⁻¹. The dose of 0.5 mg L⁻¹ of tungsten increases the SMA by 17%, but no improvement was observed with the addition of cobalt. The results suggested that TM were not bioavailable at high salinity. Accordingly, an increased COD removal was achieved by doubling the supply of TM.

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Anaerobic digester (AD) microbiomes harbor complex, interacting microbial populations to achieve biomass reduction and biogas production, however how they are influenced by operating conditions and feed sludge microorganisms remain unclear. These were addressed by analyzing the microbial communities of 90 full-scale digesters at 51 municipal wastewater treatment plants from five countries. Heterogeneity detected in community structures suggested that no single AD microbiome could be defined. Instead, the AD microbiomes were classified into eight clusters driven by operating conditions (e.g., pretreatment, temperature range, and salinity), whereas geographic location of the digesters did not have significant impacts. Comparing digesters populations with those present in the corresponding feed sludge led to the identification of a hitherto overlooked feed-associated microbial group (i.e., the residue populations). They accounted for up to 21.4% of total sequences in ADs operated at low temperature, presumably due to ineffective digestion, and as low as 0.8% in ADs with pretreatment. Within each cluster, a core microbiome was defined, including methanogens, syntrophic metabolizers, fermenters, and the newly described residue populations. Our work provides insights into the key factors shaping full-scale AD microbiomes in a global scale, and draws attentions to the overlooked residue populations.

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Methyl isobutyl ketone (MIBK) as a solvent is extensively used for the phenols extraction from the wastewater, so it is unavoidable to expose in the effluent due to the solubility and leakage problem. The present study evaluated the impact of MIBK on phenols degradation in an UASB reactor and analyzed its degradation properties. The results indicated that the continuous dosing (0.1 g L⁻¹) and impact (10 g L⁻¹) of MIBK had limited effect on phenols removal (1–2% reduction) in the UASB reactor, but the specific methanogenic activity (SMA) values of sludge decreased by 45–75% after MIBK exposure. Anaerobic degradation rate of MIBK fitted well to a pseudo-first-order kinetic equation with respect to the initial concentration of 35 mg L⁻¹ (k = 0.0115 h⁻¹, R² = 0.9664). Furthermore, the relative methane generation rate constants of MIBK were 0.00816, 0.00613, 0.00273, and 0.00207 d⁻¹ at the initial concentrations of 0.1, 0.5, 5, and 10 g L⁻¹, respectively. MIBK showed higher inhibitory effect on the methanogenesis than on phenols degradation. This study pointed out that the industrial installations should consider the influence of solvent on anaerobic treatment of phenolic wastewater.

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The cometabolism as a technique for enhancing biodegradation of pentachlorophenol (PCP) has been widely used, but the effect of dosing mode of growth substrates on the cometabolic degradation of PCP in an anaerobic system with and without the addition of ZVI is still not clear. In this study, the effects of dosing modes of sodium acetate as co-substrate, i.e., one-step pulse, evenly distributed pulse, and stepwise declining pulse, and zero-valent iron (ZVI) of 1 mg l−1 on pentachlorophenol (PCP) degradation and changes in anaerobic microbial community were examined in batch tests. The results indicated that the degradation kinetic constants of PCP were related to the dosing mode of co-substrate. The complete mineralization rates of PCP in the two groups with evenly distributed pulse mode and stepwise declining pulse mode were faster than the observed rates of the group with one-step pulse mode, according to the quantitative analysis of 2,3,5,6-tetrachlorophenol, 2,3,5-tetrachlorophenol, and 2,5-chlorophenol. In addition, there was a substantial change in the content of intracellular polysaccharide in the cometabolic process of the one-step pulse and stepwise declining pulse groups. Furthermore, the electron transport system (ETS) activities reached to the maximum values of 0.841 and 0.813 ug g−1 in these two groups with the addition of 1 g l−1 ZVI, respectively. Moreover, Alcaligenes (11.02%), Comamonas (14.58%), and Ralstonia (9.16%) were the dominant genera in the groups with the three co-substrate dosing modes, respectively. This study provides a new insight into achieving anaerobic cometabolic degradation of PCP with different dosing modes of co-substrate.@en