We present an investigation of the impact of partial coherence on optical imaging systems with the focus on whole slide imaging (WSI) systems for digital pathology. The investigation is based on the analysis of the edge response of the optical system, which gives rise to an apparent optical transfer function (OTF) that can be linked to two elementary complex functions Q and U. The function Q is directly related to the transmission cross coefficient (TCC) and can be identified with the performance function first introduced by Kintner and Sillitto. The function U depends on the TCC in a more involved way. When there are no aberrations the Q-function corresponds to the real part of the apparent OTF and the U function to the imaginary part of the apparent OTF. Close to the incoherent limit the effect of the U function is a mere shift of the edge compared to the fully incoherent case. We propose a new expression for the dependence of the depth of focus (DOF) on spatial frequency and on the partial coherence factor σ, and validate it by simulation. Partial coherence effects are investigated experimentally on a WSI system with a compact LED-based Köhler illumination unit with variable condenser NA. This unit incorporates a top hat diffuser for providing a reasonably uniform illumination field, with variations below 10% across the imaged field of view. The measurements of the apparent through-focus OTF derived from edges on a custom resolution chart for different σ were substantially in agreement with the simulations. Finding an optimal value for σ is not straightforward as lateral resolution and the level of edge ringing improve with increasing σ, whereas edge contrast and DOF improve with decreasing σ. We assess that the trade-off for the particular application of WSI systems for digital pathology is optimized for a σ value in the range of 0.55-0.75.@en

In this paper, a novel statistical approach is presented for time-of-arrival (TOA) estimation based on first path (FP) pulse detection using a sub-Nyquist sampling ultra-wide band (UWB) receiver. The TOA measurement accuracy, which cannot be improved by averaging of the received signal, can be enhanced by the statistical processing of a number of TOA measurements. The TOA statistics are modeled and analyzed for a UWB receiver using threshold crossing detection of a pulse signal with noise. The detection and estimation scheme based on the Generalized Likelihood Ratio Test (GLRT) detector, which captures the full statistical information of the measurement data, is shown to achieve accurate TOA estimation and allows for a trade-off between the threshold level, the noise level, the amplitude and the arrival time of the first path pulse, and the accuracy of the obtained final TOA.

@en

Digital pathology is based on the use of digital images of tissues for diagnosis of diseases. In the emerging clinical practice of digital pathology, images of tissue slides are acquired with a high-resolution and high-throughput automated microscope, a so called Whole Slide Imaging (WSI) system. We designed, built and characterized a modular WSI platform for conducting two- and three-dimensional brightfield microscopy, the most common modality in this field.@en

We measure the through-focus OTF of whole slide scanners for optical quality testing and monitoring. Analysis of the OTF data gives a system level evaluation of astigmatism, field curvature, chromatic aberrations, coma and spherical aberration.@en