Objective.The integration of proton beamlines with x-ray imaging/irradiation platforms has opened up possibilities for image-guided Bragg peak irradiations in small animals. Such irradiations allow selective targeting of normal tissue substructures and tumours. However, their small size and location pose challenges in designing experiments. This work presents a simulation framework useful for optimizing beamlines, imaging protocols, and design of animal experiments. The usage of the framework is demonstrated, mainly focusing on the imaging part.Approach.The fastCAT toolkit was modified with Monte Carlo (MC)-calculated primary and scatter data of a small animal imager for the simulation of micro-CT scans. The simulated CT of a mini-calibration phantom from fastCAT was validated against a full MC TOPAS CT simulation. A realistic beam model of a preclinical proton facility was obtained from beam transport simulations to create irradiation plans in matRad. Simulated CT images of a digital mouse phantom were generated using single-energy CT (SECT) and dual-energy CT (DECT) protocols and their accuracy in proton stopping power ratio (SPR) estimation and their impact on calculated proton dose distributions in a mouse were evaluated.Main results.The CT numbers from fastCAT agree within 11 HU with TOPAS except for materials at the centre of the phantom. Discrepancies for central inserts are caused by beam hardening issues. The root mean square deviation in the SPR for the best SECT (90 kV/Cu) and DECT (50 kV/Al-90 kV/Al) protocols are 3.7% and 1.0%, respectively. Dose distributions calculated for SECT and DECT datasets revealed range shifts <0.1 mm, gamma pass rates (3%/0.1 mm) greater than 99%, and no substantial dosimetric differences for all structures. The outcomes suggest that SECT is sufficient for proton treatment planning in animals.Significance.The framework is a useful tool for the development of an optimized experimental configuration without using animals and beam time.

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Land cover and land management changes (LCLMCs) play an important role in achieving low-end warming scenarios through land-based mitigation. However, their effects on moisture fluxes and recycling remain uncertain, although they have important implications for the future viability of such strategies. Here, we analyse the impact of idealized LCLMC scenarios on atmospheric moisture transport in three different Earth system model (ESMs): the Community Earth System Model (CESM), the Max Planck Institute Earth System Model (MPI-ESM), and the European Consortium Earth System Model (EC-EARTH). The LCLMC scenarios comprise of a full cropland world, a fully afforested world, and a cropland world with unlimited irrigation expansion. The effects of these LCLMC in the different ESMs are analysed for precipitation, evaporation, and vertically integrated moisture flux convergence to understand the LCLMC-induced changes in the atmospheric moisture cycle. Then, a moisture tracking algorithm is applied to assess the effects of LCLMC on moisture recycling at the local (grid cell level) and the global scale (continental moisture recycling). By applying a moisture tracking algorithm on fully coupled ESM simulations we are able to quantify the complete effects of LCLMC on moisture recycling (including circulation changes), which are generally not considered in moisture recycling studies. Our results indicate that cropland expansion is generally causing a drying and reduced local moisture recycling, while afforestation and irrigation expansion generally cause wetting and increased local moisture recycling. However, the strength of this effect varies across ESMs and shows a large dependency on the dominant driver. Some ESMs show a dominance of large-scale atmospheric circulation changes while other ESMs show a dominance of local to regional changes in the atmospheric water cycle only within the vicinity of the LCLMC. Overall, these results corroborate that LCLMC can induce substantial effects on the atmospheric water cycle and moisture recycling, both through local effects and changes in atmospheric circulation. However, more research is needed to constrain the uncertainty of these effects within ESMs to better inform future land-based mitigation strategies.@en