The Caspian Sea is an evolutionary island whose rich and endemic fauna have evolved in partial isolation over the past two million years. Baseline studies of pre-20^th Century communities are needed in order to assess the severity of the current Caspian biodiversity crisis, which mostly involves invasive species. An inventory of late Holocene shelly assemblages (c. 2000–2500 cal yr BP) from outcrops in and around Great Turali Lake (Dagestan, Russia) shows a diverse nearshore community consisting of 24 endemic Caspian species, two invasive species and two Caspian native species that lived in a shallow embayment with mesohaline salinities of circa 5–13 psu (parts per thousands). This pre-crisis Holocene Caspian mollusc community serves as a baseline against which modern mollusc diversity measurements can be evaluated. Examination of faunas from similar environments living today and in the past illustrates the dramatic changes in nearshore communities during the 20^th Century. Our study identifies a habitat that may have served as a refuge, but that is currently under threat from invasive species. The severity of the Caspian biodiversity crisis is comparable with other well-known biodiversity crises in semi-isolated ecosystems such as the cichlid fish communities of Lake Victoria, Africa.

The shallow-marine carbonate rocks of the Jandaíra Formation have been subject to significant permeability variations through time due to various events of fracturing and calcite cementation. As a consequence, the Jandaíra Formation accommodated fluid flow only during specific moments in time. We reconstructed these episodes of fluid flow based on isotope characterizations and microscope characteristics of calcite veins and host rock cements. The Jandaíra Formation, which belongs to the post-rift sequence of the Potiguar Basin in northeast Brazil, was deposited from the Turonian onward until a marine regression exposed it in the Campanian. Due to the subaerial exposure, meteoric waters flushed out marine connate waters, leading to an event of early diagenesis and full cementation of the Jandaíra Formation. Fluid flow through the resulting impermeable carbonate formation appears to be closely related to fracturing. Fracturing in the Late Cretaceous induced a drastic increase in permeability, giving rise to extensive fluid circulation. Host rock dissolution associated to the circulating fluids led to calcite vein cementation within the fracture network, causing it to regain an impermeable and sealing character. In the research area, fluid flow occurred during early burial of the Jandaíra Formation at estimated depths of 400–900 m. This study documents the first application of fluid inclusion isotope analysis on vein precipitates, which allowed full isotopic characterization of the paleo-fluids responsible for calcite vein cementation. The fluid inclusion isotope data indicate that upwelling of groundwater from the underlying Açu sandstones provided the fluids to the fracture network. In Miocene times, renewed tectonic compression of a lower intensity created a secondary fracture network in the Jandaíra Formation. The density of this fracture network, however, was too low to induce a new episode of fluid circulation. As a result, this tectonic event is associated with the development of barren extensional fractures.

Pervasive fracture networks are common in many reservoir-scale carbonate bodies even in the absence of large deformation and exert a major impact on their mechanical and flow behaviour. The Upper Cretaceous Jandaíra Formation is a few hundred meters thick succession of shallow water carbonates deposited during the early post-rift stage of the Potiguar rift (NE Brazil). The Jandaíra Formation in the present onshore domain experienced <1.5 km thermal subsidence and, following Tertiary exhumation, forms outcrops over an area of >1000 km². The carbonates have a gentle, <5⁰, dip to the NE and are affected by few regional, low displacement faults or folds. Despite their simple tectonic history, carbonates display ubiquitous open fractures, sub-vertical veins, and sub-vertical as well as sub-horizontal stylolites. Combining structural analysis, drone imaging, isotope studies and mathematical modelling, we reconstruct the fracturing history of the Jandaíra Formation during and following subsidence and analyse the impact fractures had on coeval fluid flow. We find that Jandaíra carbonates, fully cemented after early diagenesis, experienced negligible deformation during the first few hundreds of meters of subsidence but were pervasively fractured when they reached depths >400-500 m. Deformation was accommodated by a dense network of sub-vertical mode I and hybrid fractures associated with sub-vertical stylolites developed in a stress field characterised by a sub-horizontal σ₁ and sub-vertical σ₂. The development of a network of hybrid fractures, rarely reported in the literature, activated the circulation of waters charged in the mountainous region, flowing along the porous Açu sandstone underlying the Jandaíra carbonates and rising to the surface through the fractured carbonates. With persisting subsidence, carbonates reached depths of 800-900 m entering a depth interval characterised by a sub-vertical σ₁. At this stage, sub-horizontal stylolites developed liberating calcite which sealed the sub-vertical open fractures transforming them in veins and preventing further flow. During Tertiary exhumation, several of the pre-existing veins and stylolites opened and became longer, and new fractures were created typically with the same directions of the older features. The simplicity of our model suggests that most rocks in passive margin settings might have followed a similar evolution and thus display similar structures. Basin Research