K. Totlani
6 records found
1
Authored
Microbioreactors for nutrient-controlled microbial cultures
Bridging the gap between bioprocess development and industrial use
It is common practice in the development of bioprocesses to genetically modify a microorganism and study a large number of resulting mutants in order to select the ones that perform best for use at the industrial scale. At industrial scale, strict nutrient-controlled growth co ...
Fed-Batch Droplet Nanobioreactor for Controlled Growth of Cyberlindnera (Pichia) jadinii
A Proof-Of-Concept Demonstration
A key bottleneck in bioprocess development is that state-of-the-art tools used for screening of cells and optimization of cultivation conditions do not represent the conditions enforced at industrial scale. At industrial scale, cell growth is strictly controlled (“fed-batch”) ...
We developed a microfluidic droplet on-demand (DoD) generator that enables the production of droplets with a volume solely governed by the geometry of the generator for a range of operating conditions. The prime reason to develop this novel type of DoD generator is that its ro ...
We report the development of a droplet-based microfluidic device, which enables long term culturing of microorganisms inside water in oil microdroplets under semi-continuous conditions. Firstly, a microdroplet containing yeast cells is trapped on chip. After an initial incubat ...
We report the continuous production of microcapsules composed of an aqueous core and permeable hydrogel shell, made stable by the controlled photo-cross-linking of the shell of an all-aqueous double emulsion. While most previous work on water-based emulsions focused on active ...