The novel formate oxidase from Aspergillus oryzae (AoFOx) is a useful catalyst to promote H₂O₂-dependent oxyfunctionalisation reactions. In this contribution we exploit the substrate promiscuity of AoFOx to fully oxidise methanol and formaldehyde to CO₂ and drive peroxygenase-catalysed stereoselective oxyfunctionalisation reactions. The highly atom efficient H₂O₂ generation system also enabled high catalytic turnover of the peroxygenase production enzyme.

The E-factor has become an important measure for the environmental impact of (bio)chemical reactions. However, summing up the obvious wastes generated in the laboratory neglects energy-related wastes (mostly greenhouse gases) which are generated elsewhere. To estimate these wastes, we propose to extend the E-factor by an energy-term (E⁺-factor). At the example of a lab-scale enzyme fermentation, we demonstrate that the E⁺-factor can constitute a multiple of the classical E-factor and therefore must not be neglected striving for a holistic estimation of the environmental impact.