Cement-based materials (CBMs) are multiscale composites whose macroscopic properties largely depend on their micro/nanoscale features. Micro and nanomechanical properties of CBMs are typically characterized using local techniques such as nanoindentation. Compared with nanoindentation, the nanoscratch allows for continuous measurement of CBMs to acquire more comprehensive and reliable nanomechanical information, which has provided a powerful tool for the characterization of CBMs at nanoscale. However, previous reviews on the application of nanoscratch in CBMs are relatively scarce and lack detailed guidance regarding specimen preparation methods and the testing procedure. This review presents a detailed discussion of specimen preparation procedures and requirements, measurements, and data analysis methods for nanoscratch testing applied to CBMs. Then, the nanomechanical properties derived from nanoscratch tests, including hardness, friction coefficient, elastic recovery ratio and fracture properties, have been summarized and discussed. Furthermore, the current uses of nanoscratch technique in CBMs, including characterization of nanoscale micorstructure, interface, tribological features, and fracture properties, are elaborated. On the nanoscale, the nanomechanical properties are employed for phase identification and to obtain the corresponding volume fractions. In addition, nanoscratch is widely utilized to identify the width, hardness, and fracture toughness of the interfacial transition zones, and to distinguish the interface between unreacted phases and hydration products. The combination of nanoscratch and other advanced techniques, such as atomic force microscopy, backscattered electron imaging, and acoustic emission to characterize the nanoscale micorstructures of CBMs is further discussed, which contributes to improving the accuracy of nanoscratch test results and broadens their applicability. In addition, some perspectives on testing methods, data analysis, and multifunctional applications of nanoindentation technology are proposed. This review aims to assist researchers in developing robust and reliable protocols for nanoscratch testing, thereby advancing the deeper understanding of the nanoscale features of CBMs.