Microinjector

Abstract

OpenLabware is an ongoing trend to create easy to use, robust, and open source lab equipment at a fraction of the cost of commercial alternatives. One such a project is known as the OpenSpritzer designed by Forman et al. [8]. The OpenSpritzer claims to be a direct replacement of a pressure injection system (Picospritzer) that can be used to transfect zebrafish embryos with DNA encoded fluorescent proteins. By making use of an Arduino, a fast-acting solenoid, and a 3D printer an adaptation of this system was designed, modified, and built to provide a simple and consistent method of producing nanoliter droplets needed for embryonic injection. The new system proved reliable and showed a linear relationship between pressure, pulse duration, and bubble diameter. No precise volume calibration was achieved due to inconsistency in pipette tip size as well as other experimental errors. However, a graphic user interface (GUI) was produced to give greater control over the system. With the GUI a serial communication can be established between Python and the Arduino. This allows the user to precisely control pulse duration and program a sequence of pulses. When compared to the commercial alternative, the Picospritzer, this system is equal in performance and delivers greater control at a fraction of the cost.