Interactive Optoelectronics Platform for Optogenetic Applications

Abstract

This dissertation explores the development and application of an interactive optoelectronics platform designed for the precise manipulation of heart rhythm using mini/micro LEDs, primarily within the context of optogenetic applications. The research uses advancements in LED technology for biomedical engineering, focusing on the design, fabrication, and characterization of LED matrix systems. Key objectives include the development of a 48x32
mini LED matrix system, characterized for its electrical, optical, and thermal properties, and evaluated for both interactive and scripted operations. Additionally, the thesis investigates the use of this matrix for optogenetic control of cardiac activities, including optical pacing, conduction block, and the induction and termination of arrhythmias, demonstrating the system’s capability to manipulate cardiomyocytes in vitro. Furthermore, a real-time interactive optoelectronic manipulation system is developed, featuring software for real-time
control and manipulation of optical patterns, incorporating fastmanipulation frame streaming, image processing, and customized calculations. The research also extends to designing an LED matrix of human atrial surface area and the associated driver for potential clinical applications. The conclusions underscore the feasibility and effectiveness of using LED-based optoelectronic systems for precise biological control, laying the groundwork for future biomedical research and clinical therapies, and highlighting the significance of
interdisciplinary approaches in advancing medical technologies.