Are stirring and sonication pre-dispersion methods equivalent for in vitro toxicology evaluation of SiC and TiC?

Journal article (2012)

Authors

J Mejia External organisation
V Valembois External organisation
JP Piret External organisation
B Masereel External organisation
O Toussaint External organisation
J Delhalle External organisation
Z Mekhalif External organisation
S Lucas External organisation
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Published Date

2012

Language

Undefined/Unknown

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Abstract

The evolution of the particle size distribution and the surface composition of silicon carbide and titanium carbide nanoparticle (NP) dispersions were studied. The pre-dispersions were prepared using two commonly used protocols for dispersion: stirring and sonication. Two dispersants were investigated (water and Pluronic F108 1 %) at two stages: predispersion and during in vitro assays. Our data show that for each tested condition, different time-dependent results for the surface chemical composition as well as size and percentage of the agglomerates and the primary particles are observed. De-agglomeration and successive or simultaneous cleaning-wrapping cycles of the nanomaterial are observed and are related to the dispersion method and the medium as well as to the chemical stability of the NP surface. Biological response during in vitro assessment was also performed for one given pre-dispersion time condition and demonstrates that the preparation method significantly alters the results.