Synthetic Biology to Engineer Bacteriophage Genomes

Book chapter (2024)

Authors

A.R. Martins Costa BN/Stan Brouns Lab - AS
Joana Azeredo University of Minho
Diana P. Pires University of Minho
Research Group
BN/Stan Brouns Lab (AS) (TU Delft)
Copyright: © 2024 A.R. Martins Costa, Joana Azeredo, Diana Priscila Pires
DOI: https://doi.org/10.1007/978-1-0716-3523-0_17
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Published Date

2024

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Stan Brouns Lab

Abstract

Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered toward a wide range of applications, including pathogen control and detection, targeted drug delivery, or even assembly of new materials. In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes will be addressed: the bacteriophage recombineering of electroporated DNA (BRED) and the yeast-based phage-engineering platform.

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