A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring

Gaio, Nikolas; Waafi, A.; Vlaming, M.L.H.; Boschman, E.; Dijkstra, P.; Nacken, P.; Braam, S.R.; Boucsein, C.; Sarro, Pasqualina M; Dekker, Ronald

doi:10.1109/MEMSYS.2018.8346549

A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring

Conference paper (2018)

Authors

Nikolas Gaio Electronic Components, Technology and Materials -

A. Waafi Student

M.L.H. Vlaming Ncardia

E. Boschman Boschman Technologies

P. Dijkstra Philips Innovation Services

P. Nacken Ncardia

S.R. Braam Ncardia

C. Boucsein Multi Channel Systems GmbH

Pasqualina M Sarro Electronic Components, Technology and Materials -

Ronald Dekker Electronic Components, Technology and Materials -

Research Group

Electronic Components, Technology and Materials () (TU Delft)

DOI: https://doi.org/10.1109/MEMSYS.2018.8346549

Packaging Monitoring Silicon Electrodes Impedance Biomembranes Biomedical monitoring

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Published Date

2018

Language

English

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Faculty

Electrical Engineering, Mathematics and Computer Science

Department

Microelectronics

Research Group

Electronic Components, Technology and Materials

Abstract

This work presents the first multi-well plate that allows for simultaneous mechanical stimulation and electrical monitoring of multiple in-vitro cell cultures in parallel. Each well of the plate is equipped with an Organ-on-Chip (OOC) device consisting of a stretchable micro-electrode array (MEA). For the first time, a film assisted molding (FAM) process was employed to embed an OOC into a multi well plate format packaging. The functionality of the MEA in the device was assessed with electrochemical impedance spectroscopy. Moreover, the biocompatibility of the plate was demonstrated with cardiomyocytes derived from human induced pluripotent stem cells (iPSC) cultured in the wells.

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