Efficient Aerobic Oxidation of trans-2-Hexen-1-ol using the Aryl Alcohol Oxidase from Pleurotus eryngii

Journal article (2019)

Authors

T. Pedroso de Almeida BT/Biocatalysis - AS
M.M.C.H. van Schie BT/Biocatalysis - AS
A. Ma External organisation, Student
F. Tieves BT/Biocatalysis - AS
S.H.H. Younes Sohag University, BT/Biocatalysis - AS
E. Fernandez Fueyo BT/Biocatalysis - AS , BN/Greg Bokinsky Lab - AS
I.W.C.E. Arends Universiteit Utrecht
Antonio Riul University of Campinas
F. Hollmann BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2019 T. Pedroso de Almeida, M.M.C.H. van Schie, A. Ma, F. Tieves, S.H.H. Younes, E. Fernandez Fueyo, I.W.C.E. Arends, A. Riul, F. Hollmann
DOI: https://doi.org/10.1002/adsc.201801312
More Info
expand_more

Published Date

2019

Language

English

Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

The selective oxidation of trans-2-hexen-1-ol to the corresponding aldehyde using a recombinant aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. Especially using the two liquid phase system to overcome solubility and product inhibition issues enabled to achieve more than 2.200.000 catalytic turnovers for the production enzyme as well as molar product concentrations, pointing towards an economic feasible reaction.