Enantioselective Sulfoxidation of Thioanisole by Cascading a Choline Oxidase and a Peroxygenase in the Presence of Natural Deep Eutectic Solvents

Journal article (2020)

Authors

Yongru Li South China University of Technology
Yunjian Ma South China University of Technology
Peilin Li South China University of Technology
Xizhen Zhang South China University of Technology
Doris Ribitsch acib GmbH
Miguel Alcalde Institute of Catalysis, CSIC, Madrid
F. Hollmann BT/Biocatalysis - AS
Yonghua Wang South China University of Technology
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2020 Yongru Li, Yunjian Ma, Peilin Li, Xizhen Zhang, Doris Ribitsch, Miguel Alcalde, F. Hollmann, Yonghua Wang
DOI: https://doi.org/10.1002/cplu.201900751
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Published Date

2020

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

A bienzymatic cascade for selective sulfoxidation is presented. The evolved recombinant peroxygenase from Agrocybe aegeritra catalyses the enantioselective sulfoxidation of thioanisole whereas the choline oxidase from Arthrobacter nicotianae provides the H2 O2 necessary via reductive activation of ambient oxygen. The reactions are performed in choline chloride-based deep eutectic solvents serving as co-solvent and stoichiometric reductant at the same time. Very promising product concentrations (up to 15 mM enantiopure sulfoxide) and catalyst performances (turnover numbers of 150,000 and 2100 for the peroxygenase and oxidase, respectively) have been achieved.