Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation

Li, X; Ottens, M.; van Dedem, G.W.K.; van der Wielen, L.A.M.; van Leeuwen, M; van Gulik, W.M.; Heijnen, J.J.; Krommenhoek, EE; Gardeniers, Han J.G.E.; van den Berg, A

Optimizing mixing in micro-bioreactors used for high throughput screening of suitable biocatalysts in industrial fermentation

Conference paper (2005)

Authors

X Li BT/Bioprocess Engineering -

M. Ottens BT/Bioprocess Engineering -

G.W.K. van Dedem OLD BT/Analytical Biotechnology

L.A.M. van der Wielen BT/Bioprocess Engineering -

M van Leeuwen OLD BT/Cell Systems Engineering

W.M. van Gulik OLD BT/Cell Systems Engineering

J.J. Heijnen OLD BT/Cell Systems Engineering

EE Krommenhoek

Han J.G.E. Gardeniers

A van den Berg

Research Group

BT/Bioprocess Engineering () (TU Delft)

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Published Date

2005

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Bioprocess Engineering

Abstract

There is a growing interest in developing minute laboratories on a 'chip' (Lab-on-a-Chip) and micro-(bio)chemical reactors as clearly shown by the research efforts of many industrial companies and research institutes in recent years. The development of a new technological platform for fed batch fermentations in very small volume (10-300 μl) reactors in an array format will be described, which in the end will allow to improve production properties of micro organisms by rapid screening under industrially relevant conditions. One of the objectives (besides developing sensing technology) is to design and test methods to analyze and control mixing. This presentation addresses several mixing methods that can be used in micro-bioreactors. The micro-bioreactor is operated under extreme conditions like high biomass and product concentrations, high oxygen requirement and long residence time (3-4 days). With the help of Computational Fluid Dynamics simulations, the influence of the mixing method on the fermentation efficiency in the microreactor is described. These results will be supported by real-life experimental mixing data.