High-Speed Super-Resolution Imaging Using Protein-Assisted DNA-PAINT

Journal article (2020)

Authors

M. Filius BN/Chirlmin Joo Lab - AS , Kavli institute of nanoscience Delft
T.J. Cui BN/Chirlmin Joo Lab - AS , Kavli institute of nanoscience Delft
A.N. Ananth TNO, , Kavli institute of nanoscience Delft
M.W. Docter Kavli institute of nanoscience Delft, BN/Technici en Analisten
Jorrit W. Hegge Wageningen University & Research
John van der Oost Wageningen University & Research
C. Joo Kavli institute of nanoscience Delft, BN/Chirlmin Joo Lab - AS
Research Group
BN/Chirlmin Joo Lab (AS) (TU Delft)
Copyright: © 2020 M. Filius, T.J. Cui, A.N. Ananth, M.W. Docter, Jorrit W. Hegge, John van der Oost, C. Joo
DOI: https://doi.org/10.1021/acs.nanolett.9b04277
More Info
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Published Date

2020

Language

English

Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Chirlmin Joo Lab

Abstract

Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of the imager strands. Here we report on a method where imager strands are loaded into a protein, Argonaute (Ago), which allows for faster binding. Ago preorders the DNA imager strand into a helical conformation, allowing for 10 times faster target binding. Using a 2D DNA origami structure, we demonstrate that Ago-assisted DNA-PAINT (Ago-PAINT) can speed up the current DNA-PAINT technique by an order of magnitude, while maintaining the high spatial resolution. We envision this tool to be useful for super-resolution imaging and other techniques that rely on nucleic acid interactions.