Single objective tilted lightsheet for three-dimensional localization microscopy

Conference paper (2021)

Authors

S. Hung Team Carlas Smith - Mechanical, Maritime and Materials Engineering
J.P. Cnossen Team Carlas Smith - Mechanical, Maritime and Materials Engineering
D. Fan Team Carlas Smith - Mechanical, Maritime and Materials Engineering
C.S. Smith Team Carlas Smith - Mechanical, Maritime and Materials Engineering
Research Group
Team Carlas Smith (Mechanical, Maritime and Materials Engineering) (TU Delft)
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Published Date

2021

Language

English

Reuse Rights

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Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Carlas Smith

Abstract

Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.

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