Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence

Journal article (2021)

Authors

Elizabeth K. Neumann VanderBilt University
Nathan Heath Patterson VanderBilt University
More Authors... External organisation
Jamie L. Allen VanderBilt University
L.G. Migas Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Haichun Yang Vanderbilt University Medical Center
Maya Brewer Vanderbilt University Medical Center
David M G Anderson VanderBilt University
Jennifer Harvey VanderBilt University
Raymond C. Harris Vanderbilt University Medical Center
Raf Van de Plas VanderBilt University, Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Research Group
Team Raf Van de Plas (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2021 Elizabeth K. Neumann, Nathan Heath Patterson, Jamie L. Allen, L.G. Migas, Haichun Yang, Maya Brewer, David M. Anderson, Jennifer Harvey, Raymond C. Harris, Raf Van de Plas, More Authors
DOI: https://doi.org/10.1016/j.xpro.2021.100747
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Published Date

2021

Language

English

Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Raf Van de Plas

Abstract

Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high-quality and reproducible datasets suitable for cross-patient comparisons that are essential to understanding human health and disease. Moreover, each of these steps can be adapted to other organ systems or diseases, enabling additional atlas efforts.