Detection of phosphorylation post-translational modifications along single peptides with nanopores
More Info
expand_more
expand_more
Abstract
Current methods to detect post-translational modifications of proteins, such as phosphate groups, cannot measure single molecules or differentiate between closely spaced phosphorylation sites. We detect post-translational modifications at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants by controllably drawing the peptide through the sensing region of a nanopore. We discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.
Files
S41587_023_01839_z.pdf
(pdf | 5.79 Mb)
- Embargo expired in 29-12-2023