Detection of phosphorylation post-translational modifications along single peptides with nanopores

Nova, I.C.; Ritmejeris, J.; Brinkerhoff, H.D.; Koenig, T.J.R.; Gundlach, Jens H.; Dekker, C.

doi:10.1038/s41587-023-01839-z

Detection of phosphorylation post-translational modifications along single peptides with nanopores

Journal article (2023)

Authors

I.C. Nova , Kavli institute of nanoscience Delft

J. Ritmejeris , Kavli institute of nanoscience Delft

H.D. Brinkerhoff University of Washington, Kavli institute of nanoscience Delft,

T.J.R. Koenig , Kavli institute of nanoscience Delft, BN/Bionanoscience

Jens H. Gundlach University of Washington

C. Dekker Kavli institute of nanoscience Delft,

DOI: https://doi.org/10.1038/s41587-023-01839-z

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Published Date

2023

Language

English

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Abstract

Current methods to detect post-translational modifications of proteins, such as phosphate groups, cannot measure single molecules or differentiate between closely spaced phosphorylation sites. We detect post-translational modifications at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants by controllably drawing the peptide through the sensing region of a nanopore. We discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.

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