Bone tissue engineering (BTE) researches the characteristics which are needed to create the ultimate bone scaffold which enhances cell response. Limited research has been done regarding the effect of the Poisson’s ratio on the scaffold-cell interaction. In this graduation project we therefore explore the cell response on scaffolds with a different value of the Poisson’s ratio. Various meta-biomaterials were designed, manufactured, mechanically tested and the response on pre-osteoblasts (MC3T3-E1) was explored. The first experiment was performed at mesoscale. The meta-biomaterials, with cells, were evaluated with SEM imaging, presto blue and ARS staining. The second experiment was performed at micro-scale. The results of this experiment were evaluated with SEM imaging, actin staining and Runx2 staining. It was concluded that the auxetic meta-biomaterial, with negative Poisson’s ratio, high porosity and high stiffness, showed an enhancement of the cell response. However, this could not be confirmed by the 2D SEM images. A potential application for the meta-biomaterial that enhances the cell response is implementing this meta-biomaterial in a design for the surface of an implant to generate fast bone ingrowth.