Recovering materials with high value and increasing market demand from sewage and/or sludge is becoming more attractive than recovering traditional resources such as nutrients and biogas. Sulfated polysaccharides (SPs) are valuable and scarce raw materials that can only be produced from marine algae and a few types of animal tissues. This study evaluated if SPs are present in activated sludge obtained from saline sewage with a high level of sulfates present. The presence of SPs-containing extracellular polymeric substances (EPS) was confirmed and quantified for both sludge from lab-scale reactors and full-scale plants for the first time. SPs in the sludge of a lab-scale reactor operated under alternating aerobic/anoxic conditions with 500 mg/L sulfate in the influent (which is typical of Hong Kong saline sewage) reached 342.8 ± 0.3% mg/gVSS, and sludge taken from a full-scale saline wastewater treatment plant (WWTP) contained 418.1 ± 0.4% mg/gVSS of SPs. Purity of the extracted SPs was comparable to that of commercial industrial-grade products. Key bioactivities of SPs (i.e. fucoidan, carrageenan and heparin), namely anti-angiogenesis, anticoagulant and antioxidant, were confirmed after extraction and purification. Interestingly, operating conditions had a strong influence on the contents and types of SPs synthesized in sludge as well as its bioactivities. Although the detailed synthetic pathways of SPs in activated sludge remain unclear, the current study has made a first attempt to recover a high–value scarce resource from biological wastewater treatment.

Stabilization of waste activated sludge (WAS) is an essential step for the disposal or reuse. In this study, WAS stabilization via electrochemical pretreatment (EPT) at 0–15V was evaluated for simultaneous dewaterability enhancement, pathogen removal and H₂S mitigation. The mechanism underlying EPT was investigated and discussed based on the changes in the physicochemical (e.g., particle size, zeta potential, hydrophobicity and extracellular polymeric substances) and biological characteristics (i.e. cell morphology, and distribution and percentages of live/dead cells) of WAS with different EPT voltages. The results revealed that EPT disintegrated WAS flocs and disrupted the cell walls leading to a reduction in particle size (by up to 50%), increased release of extracellular and intracellular substances (by up to 4 times) to facilitate WAS stabilization. With EPT at 15V, the capillary suction time of WAS decreased by 42%, and the concentrations of E. coli and indicator pathogens (Salmonella spp. and Streptococcus faecalis) fell by nearly 5 log₁₀ reaching U.S. EPA hygienization levels. Furthermore, EPT at 12V or higher suppressed the amounts of dissolved sulfide and H₂S_(g) produced from the WAS under anaerobic conditions by over 99%. This study demonstrates the feasibility of EPT for simultaneous WAS dewaterability enhancement, pathogen inactivation and H₂S mitigation, providing a one-step alternative for sludge stabilization.

Sulfide prevails in both industrial and municipal waste streams and is one of the most troublesome issues with waste handling. Various technologies and strategies have been developed and used to deal with sulfide for decades, among which biological means make up a considerable portion due to their low operation requirements and flexibility. Sulfur bacteria play a vital role in these biotechnologies. In this article, conventional biological approaches dealing with sulfide and functional microorganisms are systematically reviewed. Linking the sulfur cycle with other nutrient cycles such as nitrogen or phosphorous, and with continued focus of waste remediation by sulfur bacteria, has led to emerging biotechnologies. Furthermore, opportunities for energy harvest and resource recovery based on sulfur bacteria are also discussed. The electroactivity of sulfur bacteria indicates a broad perspective of sulfur-based bioelectrochemical systems in terms of bioelectricity production and bioelectrochemical synthesis. The considerable PHA accumulation, high yield and anoxygenic growth conditions in certain phototrophic sulfur bacteria could provide an interesting alternative for bioplastic production. In this review, new merits of biological sulfide oxidation from a traditional environmental management perspective as well as a waste to resource perspective are presented along with their potential applications.