As in many countries, in The Netherlands governmental organisations are acquiring 3D city models to support their public tasks. However, this is still being done within individual organisation, resulting in differences in 3D city models within one country and sometimes covering the same area: i.e. differences in data structure, height references used, update cycle, data quality, use of the 3D data etc. In addition, often only large governmental organisations can afford investing in 3D city models (and the required knowledge) and not small organisations, like small municipalities. To address this problem, the Dutch Kadaster is collaborating with the 3D Geoinformation research group at TU Delft to generate and disseminate a 3D city model covering the whole of the Netherlands and to do this in a sustainable manner, i.e. with an implementation that ensures periodical updates and that aligns with the 3D city models of other governmental organisations, such as large cities. This article describes the workflow that has been developed and implemented.

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Successful stem cell therapy after acute myocardial infarction (AMI) is hindered by lack of engraftment of sufficient stem cells at the site of injury. We designed a novel technique to overcome this problem by assembling stem cell-microbubble complexes, named 'StemBells'. StemBells were assembled through binding of dual-targeted microbubbles (~3μm) to adipose-derived stem cells (ASCs) via a CD90 antibody. StemBells were targeted to the infarct area via an ICAM-1 antibody on the microbubbles. StemBells were characterized microscopically and by flow cytometry. The effect of ultrasound on directing StemBells towards the vessel wall was demonstrated in an in vitro flow model. In a rat AMI-reperfusion model, StemBells or ASCs were injected one week post-infarction. A pilot study demonstrated feasibility of intravenous StemBell injection, resulting in localization in ICAM-1-positive infarct area three hours post-injection. In a functional study five weeks after injection of StemBells cardiac function was significantly improved compared with controls, as monitored by 2D-echocardiography. This functional improvement neither coincided with a reduction in infarct size as determined by histochemical analysis, nor with a change in anti- and pro-inflammatory macrophages. In conclusion, the StemBell technique is a novel and feasible method, able to improve cardiac function post-AMI in rats.@en