Diclofenac is a worldwide consumed drug included in the watch list of substances to be monitored according to the European Union Water Framework Directive (Directive 2013/39/EU). Aerobic granular sludge sequencing batch reactors (AGS-SBR) are increasingly used for wastewater treatment but there is scant information on the fate and effect of micropollutants to nutrient removal processes. An AGS-SBR fed with synthetic wastewater containing diclofenac was bioaugmented with a diclofenac degrading bacterial strain and performance and microbial community dynamics was analysed. Chemical oxygen demand, phosphate and ammonia removal were not affected by the micropollutant at 0.03 mM (9.54 mg L^–1). The AGS was able to retain the degrading strain, which was detected in the sludge throughout after augmentation. Nevertheless, besides some adsorption to the biomass, diclofenac was not degraded by the augmented sludge given the short operating cycles and even if batch degradation assays confirmed that the bioaugmented AGS was able to biodegrade the compound. The exposure to the pharmaceutical affected the microbial community of the sludge, separating the two first phases of reactor operation (acclimatization and granulation) from subsequent phases. The AGS was able to keep the bioaugmented strain and to maintain the main functions of nutrient removal even through the long exposure to the pharmaceutical, but combined strategies are needed to reduce the spread of micropollutants in the environment.

Aerobic Granular Sludge (AGS) has been successfully applied for carbon, nitrogen and phosphorous removal from wastewaters, in a single tank, reducing the space and energy requirements. This is especially beneficial for, often space restricted, industrial facilities. Moreover, AGS holds a promise for the toxic pollutants removal, due to its layered and compact structure and the bacteria embedding in a protective extracellular polymeric matrix. These outstanding features contribute to AGS tolerance to toxicity and stability. Strategies available to deal with toxic compounds, namely granulation with effluents containing toxics and bioaugmentation, are addressed here. Different applications for the toxics/micropollutants removal through biosorption and/or biodegradation are presented, illustrating the technology versatility. The anthropogenic substances effects on system performance and bacterial populations established within AGS are also addressed. Combination of contaminants removal to allow water discharge, and simultaneous valuable products recovery are presented as final remark.