Thermal drying is an effective sludge treatment method for dealing with large volumes of sludge. Microwave (MW) technology has been proposed as an effective and efficient technology for sludge drying. The physical-chemical properties of the sludge depend both on the origin of the sludge, as well as on the treatment process at which the sludge has been exposed. The physical-chemical properties of the sludge affect the performance and the subsequent valorisation and management of the sludge. This study evaluated the effect of certain physical-chemical properties of the sludge (moisture content, organic content, calorific value, porosity, hydrophobicity, and water-sludge molecular interaction, among others) on the MW sludge drying and energy performance. Four different types of sludge were evaluated collected from municipal wastewater treatment plants and septic tanks. The performance of the MW system was assessed by evaluating the sludge drying rates, exposure times, energy efficiencies and power input consumed by the MW system and linking the MW drying performance to the sludge physical-chemical properties. The results confirmed that MW drying substantially extends the constant drying period associated with unbound water evaporation, irrespective of the sludge sample evaluated. However, the duration and intensity were determined to depend on the dielectric properties of the sludge, particularly on the distribution of bound and free water. Sludge samples with a higher amount of free and loosely bound water absorbed and converted MW energy into heat more efficiently than sludge samples with a lower amount of free water. As a result, the sludge drying rates increased and the constant drying rate period prolonged; hence, leading to an increase in MW drying energy efficiency. The availability of free and loosely bound water molecules was favoured when hydrophobic compounds, e.g., oils and fats, were present in the sludge.

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This study evaluated the effects of sludge retention time (SRT) on the membrane filtration performance of an anaerobic membrane bioreactor (AnMBR) fed lipid-rich synthetic dairy wastewater. The membrane filtration performance was evaluated in two AnMBR systems operated at two different SRTs, i.e., 20 and 40 days. For the AnMBR operated at 40 days, SRT exhibited worse membrane filtration performance characterized by operational transmembrane pressures (TMP) exceeding the maximum allowed value and high total resistances to filtration (Rtotal). The sludge in the two reactors evaluated at the different SRTs showed similar sludge filterability properties. However, the sludge in the reactor operated at 40 days SRT was characterized by exhibiting the highest concentrations of: (i) total suspended solids (TSS), (ii) small-sized particles, (iii) extracellular polymeric substances (EPS), (iv) soluble microbial products (SMP), (v) fats, oils and grease (FOG), and (vi) long-chain fatty acids (LCFA). The cake layer resistance was the major contributor to the overall resistance to filtration. The high TSS concentration observed in the AnMBR systems apparently contributed to a less permeable cake layer introducing a negative effect on the membrane filtration performance.@en

The provision of effective sanitation strategies has a significant impact on public health. However, the treatment of septic sludge still presents some challenges worldwide. Consequently, innovative technologies capable of an effective and efficient sludge treatment, mostly at a decentralized level, are in high demand to improve sanitation provision. To address this problem, this study evaluates a novel semi-decentralised mobile faecal sludge treatment system, the pilot-system for which consists of a combination of several individual processes including mechanical dewatering (MD), microwave (MW) drying, and membrane filtration (ultrafiltration [UF] and reverse osmosis [RO]). The system evaluation was carried out by treating raw, partially digested faecal sludge (FS) from septic tanks—hence, septic sludge (SS)—in the Jordan Valley, Jordan. The pilot-scale system exhibited an effective and flexible treatment performance for (i) sanitizing faecal sludge and related liquid streams (MW and UF); (ii) reducing the treated sludge mass (and sludge volume) (MD and MW); and (iii) producing a high-quality treated liquid stream ideal for water reclamation applications (UF and RO). The MD process removed approximately 99% of the initial SS water content. The MW drying system completely removed E. coli and dehydrated the dewatered sludge at low energy expenditures of 0.75 MJ kg⁻¹ and 5.5 MJ kg⁻¹, respectively. Such energy expenditures can be further reduced by approximately 40% by recovering energy in the condensate and burning the dried sludge, which can then be reused inland applications. The membrane filtration system (UF and RO) was able to produce high-quality treated water that is ideal for the water reuse applications that irrigation requires, as well as meeting the Jordanian standard 893/2006. In addition, the system can also be powered by renewable energy sources, such as photovoltaic energy. Therefore, this research demonstrates that the evaluated semi-decentralised mobile system is technically feasible for the in situ treatment of SS (sanitization and dehydration), while also being effective for simultaneously recovering valuable resources, such as energy, water, and nutrients.

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Sewage sludge management and treatment can represent up to approximately 30% of the overall operational costs of a wastewater treatment plant. Microwave (MW) drying has been recognized as a feasible technology for sludge treatment. However, MW drying systems exhibit high energy expenditures due to: (i) unnecessary heating of the cavity and other components of the system, (ii) ineffective extraction of the condensate from the irradiation cavity, and (iii) an inefficient use of the microwave energy, among others issues. This study investigated the performance of a novel pilot-scale MW system for sludge drying, specifically designed addressing the shortcomings previously described. The performance of the system was assessed drying municipal centrifuged wasted activated sludge at MW output powers from 1 to 6 kW and evaluating the system's drying rates and exposure times, specific energy outputs, MW generation efficiencies, overall energy efficiencies, and specific energy consumption. The results indicated that MW drying significantly extends the duration of the constant rate drying period associated with the evaporation of the unbound sludge water, a phase associated with low energy input requirement for evaporating water. Moreover, the higher the MW output power, the higher the sludge power absorption density, and the MW generation efficiency. MW generation efficiencies of up to 70% were reported. The higher the power absorption density, the lower the chances for energy losses in the form of reflected power and/or energy dissipated into the MW system. Specific energy consumptions as low as 2.6 MJ L⁻¹ (0.74 kWh L⁻¹) could be achieved, well in the range of conventional thermal dryers. The results obtained in this research provide sufficient evidence to conclude that the modifications introduced to the novel pilot-scale MW system mitigated the shortcomings of existing MW systems, and that the technology has great potential to effectively and efficiently drying municipal sewage sludge.

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The aim of this study was to evaluate the effectiveness of the novel aerobic granular sludge (AGS) wastewater treatment technology in removing faecal indicator organisms (FIOs) compared to the conventional activated sludge (CAS) treatment system. The work was carried out at two full-scale wastewater treatment plants (WWTP) in the Netherlands, Vroomshoop and Garmerwolde. Both treatment plants have a CAS and AGS system operated in parallel. The parallel treatment lines are provided with the same influent wastewater. The concentrations of the measured FIOs in the influent of the two WWTPs were comparable with reported literature values as follows: F-specific RNA bacteriophages at 10⁶ PFU/100 mL, and Escherichia coli (E. coli), Enterococci, and Thermotolerant coliforms (TtC) at 10⁵ to 10⁶ CFU/100 mL. Although both systems (CAS and AGS) are different in terms of design, operation, and microbial community, both systems showed similar FIOs removal efficiency. At the Vroomshoop WWTP, Log₁₀ removals for F-specific RNA bacteriophages of 1.4 ± 0.5 and 1.3 ± 0.6 were obtained for the AGS and CAS systems, while at the Garmerwolde WWTP, Log₁₀ removals for F-specific RNA bacteriophages of 1.9 ± 0.7 and 2.1 ± 0.7 were found for the AGS and CAS systems. Correspondingly, E. coli, Enterococci, and TtC Log₁₀ removals of 1.7 ± 0.7 and 1.1 ± 0.7 were achieved for the AGS and CAS systems at Vroomshoop WWTP. For Garmerwolde WWTP Log₁₀ removals of 2.3 ± 0.8 and 1.9 ± 0.7 for the AGS and CAS systems were found, respectively. The measured difference in removal rates between the plants was not significant. Physicochemical water quality parameters, such as the concentrations of organic matter, nutrients, and total suspended solids (TSS) were also determined. Overall, it was not possible to establish a direct correlation between the physicochemical parameters and the removal of FIOs for any of the treatment systems (CAS and AGS). Only the removal of TSS could be positively correlated to the E. coli removal for the AGS technology at the evaluated WWTPs.

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The co-treatment of two synthetic faecal sludges (FS-1 and FS-2) with municipal synthetic wastewater (WW) was evaluated in an aerobic granular sludge (AGS) reactor. After characterisation, FS-1 showed the following concentrations, representative for medium-strength FS: 12,180 mg TSS L⁻¹, 24,300 mg total COD L⁻¹, 93.8 mg PO₃-P L⁻¹, and 325 mg NH₄-N L⁻¹. The NO₃-N concentration was relatively high (300 mg L⁻¹). For FS-2, the main difference with FS-1 was a lower nitrate concentration (18 mg L⁻¹). The recipes were added consecutively, together with the WW, to an AGS reactor. In the case of FS-1, the system was fed with 7.2 kg total COD m⁻³d⁻¹ and 0.5 kg Nitrogen m⁻³d⁻¹. Undesired denitrification occurred during feeding and settling resulting in floating sludge and wash-out. In the case of FS-2, the system was fed with 8.0 kg total COD m⁻³d⁻¹ and 0.3 kg Nitrogen m⁻³d⁻¹. The lower NO₃-N concentration in FS-2 resulted in less floating sludge, a more stabilised granular bed and better effluent concentrations. To enhance the hydrolysis of the slowly biodegradable particulates from the synthetic FS, an anaerobic stand-by period was added and the aeration period was increased. Overall, when compared to a control AGS reactor, a lower COD consumption (from 87 to 35 mg g⁻¹ VSS h⁻¹), P-uptake rates (from 6.0 to 2.0 mg P g VSS⁻¹ h⁻¹) and NH₄-N removal (from 2.5 to 1.4 mg NH₄-N g VSS⁻¹ h⁻¹) were registered after introducing the synthetic FS. Approximately 40% of the granular bed became flocculent at the end of the study, and a reduction of the granular size accompanied by higher solids accumulation in the reactor was observed. A considerable protozoa Vorticella spp. bloom attached to the granules and the accumulated particles occurred; potentially contributing to the removal of the suspended solids which were part of the FS recipe.

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In this study, the impact of applied solids retention time (SRT) on the biological performance of an anaerobic membrane bioreactor (AnMBR) treating synthetic dairy wastewater with high lipid content was assessed. Two side-stream AnMBR systems were operated at an SRT of 20 and 40 days (R20 and R40, respectively), equipped with an inside-out tubular membrane operated in cross-flow mode under full-scale operational conditions, i.e. crossflow velocity, transmembrane pressure, membrane flux. Successful operation was achieved and removal efficiencies of both reactors were up to 99% applying an organic loading rate (OLR) of 4.7 g COD L⁻¹ d⁻¹. No precipitation of lipids was observed throughout the operational period, keeping the lipids available for the anaerobic degradation. Long chain fatty acid (LCFA) accumulation was very modest and amounted 148 and 115 mg LCFA-COD per gram of volatile suspended solids (VSS) for R20 and R40, respectively. At an SRT of 40 days, a slightly better biological conversion was obtained. Periodically performed specific methanogenic activity (SMA) tests showed stabilization of the SMA for R40 sludge, whereas for R20 sludge the SMA continued to decrease. This study revealed a more stable reactor performance operating the AnMBR at an SRT of 40 days compared to 20 days.

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The performance of a pilot-scale superoxygenation system was evaluated in clean water and mixed liquor. A mass balance was applied over the pilot-scale system to determine the overall oxygen mass transfer rate coefficient (K_La, h⁻¹), the standard oxygen transfer rate (SOTR, kg O₂ d⁻¹), and the standard oxygen transfer efficiency (SOTE, %). Additionally, the alpha factor (α) was determined at a mixed liquor suspend solids (MLSS) concentration of approximately 5 g L⁻¹. SOTEs of nearly 100% were obtained in clean water and mixed liquor. The results showed that at higher oxygen flowrates, higher transfer rates could be achieved; this however, at expenses of the transfer efficiency. As expected, lower transfer efficiencies were observed in mixed liquor compared to clean water. Alpha factors varied between 0.6 and 1.0. However, values of approximately 1.0 can be obtained in all cases by fine tuning the oxygen flowrate delivered to the system.

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An experimental prototype of the eSOS (emergency Sanitation Operation System) Smart Toilet^® was evaluated in an emergency settlement in the Philippines. The toilet was equipped with sensors and information communication technologies (ICT) for an efficient operation in an emergency setting. The field testing aimed at evaluating the toilet's service capacity related to the user frequency/intensity obtaining insight on the usage patterns in an actual post emergency situation. In addition, the novel features and functionality of the toilet were assessed. Operational performance of the toilet was assessed based on data collected from nearly 700 users within a 7-weeks period. The eSOS Smart Toilet has been properly operating during the evaluation period. A methodology to distinguish defecation and urination activities was developed based on determining discharges to faeces and urine tank. The toilet achieved up to 97% savings on water consumption compared to conventional toilets. The application of sensors and ICT features, combined with manually obtained data informed comprehensive usages data e.g. 62% of identified users were female users, 40% children, and 60% of the visits were for urination and 40% and for defecation. The accumulation of urine, faeces and grey water was measured to allow for a responsive maintenance resulting in optimized operation and increased interest to use the toilet. The field evaluation generated ideas for further improvements in terms of cost savings, services, and an overall vision for sustainability.

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Applying ozone to the return flow in an activated sludge (AS) process is a way for reducing the residual solids production. To be able to extend the activated sludge models to the ozone-AS process, adequate prediction of the tri-atoms effects on the particulate COD fractions is needed. In this study, the biomass inactivation, COD mineralization, and solids dissolution were quantified in batch tests and dose-response models were developed as a function of the reacted ozone doses (ROD). Three kinds of model-sludge were used. S1 was a lab-cultivated synthetic sludge with two components (heterotrophs X_H and X_P). S2 was a digestate of S1 almost made by the endogenous residues, X_P. S3 was from a municipal activated sludge plant. The specific ozone uptake rate (SO₃UR, mgO₃/gCOD.h) was determined as a tool for characterizing the reactivity of the sludges. SO₃UR increased with the X_H fraction and decreased with more X_P. Biomass inactivation was exponential (e^−β.ROD) as a function of the ROD doses. The percentage of solids reduction was predictable through a linear model (C_Miner + Y_sol ROD), with a fixed part due to mineralization (C_Miner) and a variable part from the solubilization process. The parameters of the models, i.e. the inactivation and the dissolution yields (β, 0.008–0.029 (mgO₃/mgCOD_ini)⁻¹ vs Y_sol, 0.5–2.8 mg COD_sol/mgO₃) varied in magnitude, depending on the intensity of the scavenging reactions and potentially the compactness of the flocs for each sludge.

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Reducing the footprint requirements of membrane bioreactors (MBR)s can both decrease the surface area needs for new wastewater treatment plants (WWTP)s, and increase the treatment capacities of existing WWTPs at a given surface area. In addition, it may promote the development of movable/portable containerized MBRs for a diverse range of wastewater treatment applications. Applications may include the provision of municipal/industrial wastewater treatment in remote areas without sewerage, and the provision of sanitation services under challenging site-specific conditions such as after the occurrence of a human-made or a natural disaster. The reduction of the footprint requirements of MBRs is constrained by the maximum amount of biomass that can be accommodated in the aerobic basin. The biomass concentration is mainly limited by the extremely low oxygen transfer efficiency (OTE) experienced by conventional aeration bubble diffuser systems at mixed liquor total suspended solids (MLSS) concentrations higher than 20 g L^-1. Another potential limitation for the operation of MBRs at such high MLSS concentrations is the reduction on the membrane permeability due to excessive fouling. A pilot MBR with a treatment capacity of one m³ d^-1 was installed at the research hall facilities at the Harnaschpolder wastewater treatment plant in Delft, The Netherlands. The MBR was operated at MLSS concentrations of up to 28 g L^-1 at sludge retention times (SRT)s ranging from 30 to 35 days. The MBR was provided with a Speece cone concentrated oxygen delivery system to overcome the oxygen transfer limitations of conventional bubble diffuser aeration systems at high MLSS concentrations. The MBR performance was evaluated by monitoring the influent and effluent water quality, the membrane permeability, the sludge filterability, the dissolved oxygen (DO) concentration, and the oxygen uptake rate (OUR). The Speece cone proved to be effective in delivering enough oxygen to maintain DO concentrations in the MBR of approximately 2 mg L^-1 at MLSS concentrations of up to 22 g L^-1. OUR values above 200 mg L^-1 h^-1 were observed at 14 g L^-1 MLSS and higher than 300 mg L^-1 h^-1 at 22 g L^-1 MLSS. The MBR exhibited chemical oxygen demand (COD) removal efficiencies of up to 99% even at a hydraulic retention time (HRT) as low as 3.7 h. A reduction in permeability from 33 to 11 lmh bar^-1 was observed when the MLSS concentrations increased from 18.7 to 27.8 g L^-1. Sludge filterability values expressed as the added resistance (δR₂₀) fell in the range of "poor filterability" for all the evaluated operational conditions; however, a lower filtration resistance in the range of "moderate filterability" at approximately 23 g L^-1 MLSS was noticed. The experimental results suggest that at the evaluated experimental conditions the existent limitations on poor oxygen transfer and low permeability when operating a MBR at high MLSS concentrations can be overcome; therefore, the footprint requirements of MBR systems may be further reduced.

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Eutrophication episodes have been recently observed in the Santa Lucia river basin (SLRB) in Uruguay, the main drinking water source for approximately 60% of the Uruguayan population. The local environmental authorities have been strengthening the discharge standards for that particular river basin. There are several industries currently discharging their wastewater directly into the SLRB; some of these industries are required to upgrade their current wastewater treatment systems to comply with the new regulations. This study evaluated the performance of a membrane bioreactor (MBR) on dairy wastewater as a potential treatment technology for fulfilling the new discharge standards. A pilot MBR was placed at the dairy industry wastewater treatment system at two different locations: (i) receiving the wastewater from the industrial process after passing through a grease removal pond (high load stream); and (ii) receiving the wastewater after passing through the grease removal pond and an anaerobic pond (low load stream). The pilot MBR was operated at the following conditions for approximately four months: total sludge retention, hydraulic retention time (HRT) of 25 h, an average influent flow rate of 1.3 m³ day^-1, and at two different average chemical oxygen demand (COD) influent concentrations: 1300 mg L^-1 (high load stream) and 385 mg L^-1 (low load stream). The average reported removal efficiencies on COD, biological oxygen demand (BOD), and ammonium (NH₄-N) were 94.1, 98.1, and 99.6%, respectively. In addition, it was observed that for a COD/N ratio above 10, total nitrogen (TN) and total phosphorous (TP) were well removed with average removal efficiencies of 93.1 and 91.0%, respectively. The MBR effluent met the new Uruguayan standards for discharging into the SLRB, and it can be further considered for water reuse at the industrial process. Moreover, a financial feasibility study was carried out for the implementation of a full scale MBR at the existing dairy facility. The results of the feasibility study suggested to accept the investment for the implementation of the MBR technology at the dairy industry. The results of the feasibility analysis considered the high impact of penalties and fines imposed by the local government to the industry when not complying with the effluent discharge standards, as well as the critical situation regarding eutrophication of the SLRB while being the most important source for drinking water in Uruguay.

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An eSOS (emergency Sanitation Operation System) Smart Toilet experimental prototype, aimed at improving the provision of safe sanitation in emergency settings, was field tested in a temporary settlement in Tacloban City, Philippines. The design, usage, and user acceptance of the toilet were all evaluated. Quantitative and qualitative data were collected through interviews and questionnaires, supported by the research-team’s observations. The survey results indicated that 98% of users (both first-time users and those who tried it a few times) intended to use the toilet again. There were more features that the users liked than disliked. The in-built water supply and user-operated smart toilet features were liked, but the bad smell was disliked. User-operated smart features were an important factor in user acceptance although they were not the main incentives. Key recommendations are to improve the toilet’s design to address the odor and cleanliness issues, make handwashing more convenient, and lower the height of the toilet bowl.

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The objective of this study was to investigate the ability of a culture highly enriched with the polyphosphate-accumulating organism, “Candidatus Accumulibacter phosphatis” clade IIC, to adjust their metabolism to different phosphate availabilities. For this purpose the biomass was cultivated in a sequencing batch reactor with acetate and exposed to different phosphate/carbon influent ratios during six experimental phases. Activity tests were conducted to determine the anaerobic kinetic and stoichiometric parameters as well as the composition of the microbial community. Increasing influent phosphate concentrations led to increased poly-phosphate content and decreased glycogen content of the biomass. In response to higher biomass poly-phosphate content, the biomass showed higher specific phosphate release rates. Together with the phosphate release rates, acetate uptake rates also increased up to an optimal poly-phosphate/glycogen ratio of 0.3 P-mol/C-mol. At higher poly-phosphate/glycogen ratios (obtained at influent P/C ratios above 0.051 P-mol/C-mol), the acetate uptake rates started to decrease. The stoichiometry of the anaerobic conversions clearly demonstrated a metabolic shift from a glycogen dominated to a poly-phosphate dominated metabolism as the biomass poly-phosphate content increased. FISH and DGGE analyses confirmed that no significant changes occurred in the microbial community, suggesting that the changes in the biomass activity were due to different metabolic behavior, allowing the organisms to proliferate under conditions with fluctuating phosphate levels.@en

Large volumes of sludge are produced from onsite sanitation systems in densely populated areas (e.g. slums and emergency settlements) and wastewater treatment facilities that contain high amounts of pathogens. There is a need for technological options which can effectively treat the rapidly accumulating sludge under these conditions. This study explored a pilot-scale microwave (MW) based reactor as a possible alternative for rapid sludge treatment. The reactor performance was examined by conducting a series of batch tests using centrifuged waste activated sludge (C-WAS), non-centrifuged waste activated sludge (WAS), faecal sludge (FS), and septic tank sludge (SS). Four kilograms of each sludge type were subjected to MW treatment at a power of 3.4 kW for various time durations ranging from 30 to 240 min. During the treatment the temperature change, bacteria inactivation (E. coli, coliforms, Staphylococcus aureus, and enterococcus faecalis) and sludge weight/volume reduction were measured. Calorific values (CV) of the dried sludge and the nutrient content (total nitrogen (TN) and total phosphorus (TP)) in both the dried sludge and the condensate were also determined. It was found that MW treatment was successful to achieve a complete bacterial inactivation and a sludge weight/volume reduction above 60%. Besides, the dried sludge and condensate had high energy (≥ 16 MJ/kg) and nutrient contents (solids; TN ≥ 28 mg/g TS and TP ≥ 15 mg/g TS; condensate TN ≥ 49 mg/L TS and TP ≥ 0.2 mg/L), having the potential to be used as biofuel, soil conditioner, fertilizer, etc. The MW reactor can be applied for the rapid treatment of sludge in areas such as slums and emergency settlements.

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Toilet facilities in highly dense areas such as the slum and emergency settlements fill up rapidly; thus, requiring frequent emptying. Consequently, big quantities of fresh faecal sludge (FS) containing large amounts of pathogens are generated. Fast and efficient FS treatment technologies are therefore required for safe treatment and disposal of the FS in such conditions. This study explores the applicability of a microwave (MW) technology for the treatment of fresh FS obtained from urine-diverting dry toilets placed in slum settlements in Nairobi, Kenya. Two sample fractions containing 100 g and 200 g of FS were exposed to MW irradiation at three input MW power levels of 465, 1085 and 1550 W at different exposure times ranging from 0.5 to 14 min. The variation in the FS temperature, pathogen reduction via the destruction of E. coli and Ascaris lumbricoides eggs, and vol/wt reduction were measured during the MW treatment. It was demonstrated that the MW technology can rapidly and efficiently achieve complete reduction of E. coli and Ascaris lumbricoides eggs, and over 70% vol/wt reduction in the fresh FS. Furthermore, the successful evaluation of the MW technology under real field conditions demonstrated that MW irradiation can be applied for rapid treatment of fresh FS in situations such as urban slum and emergency conditions.

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P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available.

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Ultraviolet germicidal (short wavelength UV-C) light was studied as surface disinfectant in an Emergency Sanitation Operation System^® smart toilet to aid to the work of manual cleaning. The UV-C light was installed and regulated as a self-cleaning feature of the toilet, which automatically irradiate after each toilet use. Two experimental phases were conducted i.e. preparatory phase consists of tests under laboratory conditions and field testing phase. The laboratory UV test indicated that irradiation for 10 min with medium–low intensity of 0.15–0.4 W/m² could achieve 6.5 log removal of Escherichia coli. Field testing of the toilet under real usage found that UV-C irradiation was capable to inactivate total coliform at toilet surfaces within 167-cm distance from the UV-C lamp (UV-C dose between 1.88 and 2.74 mW). UV-C irradiation is most effective with the support of effective manual cleaning. Application of UV-C for surface disinfection in emergency toilets could potentially reduce public health risks.

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