Monitoring the microparticle transfer process in wastewater treatment systems is crucial for improving treatment performance. Supervised deep learning methods show high performance to automatically detect particles, but they rely on vast amounts of labeled data for training. To overcome this issue, we proposed a semi-supervised learning (SSL) method based on the Simple framework for Contrastive Learning of visual Representations (SimCLR), to detect microparticles free from sludge and attached to sludge. First, we pre-trained a ResNet50 backbone by SimCLR, to extract features from much unlabeled data (1,000 images). Then, we constructed a Mask R-CNN architecture based on the pre-trained ResNet50, and fine-tuned it on a small quantity of labeled data (≈200 images with ≈600 annotated particles) in supervised learning fashion. We showcased its performance and practical applicability for microscopy images obtained from the water lab of TU Delft. The results demonstrate that the SSL methods obtain a significant improvement in mean average precision of up to 5% compared to the conventional supervised learning method, when a limited amount of labeled data is available (e.g., 91 labeled images). Furthermore, these methods improve the average precision for detecting attached particles by over 12%. With the detection results from the SSL methods, we measured the attachment efficiency of microparticles to sludge under varying mixed liquor suspended solids concentration and aeration intensity. The precise measurements demonstrate the effectiveness and practical applicability of the SSL method in facilitating long-term monitoring of particle transfer processes in biological wastewater treatment systems.

Investigating the interaction between influent particles and biomass is basic and important for the biological wastewater treatment. The micro-level methods allow for this, such as the microscope image analysis method with the conventional ImageJ processing software. However, these methods are cost and time-consuming, and require a large amount of work on manual parameter tuning. To deal with this problem, we proposed a deep learning (DL) method to automatically detect and quantify microparticles free from biomass and entrapped in biomass from microscope images. Firstly, we introduced a “TU Delft-Interaction between Particles and Biomass” dataset containing labeled microscope images. Then, we built DL models using this dataset with seven state-of-the-art model architectures for a instance segmentation task, such as Mask R-CNN, Cascade Mask R-CNN, Yolact and YOLOv8. The results show that the Cascade Mask R-CNN with ResNet50 backbone achieves promising detection accuracy, with a mAP50_box and mAP50_mask of 90.6 % on the test set. Then, we benchmarked our results against the conventional ImageJ processing method. The results show that the DL method significantly outperforms the ImageJ processing method in terms of detection accuracy and processing cost. The DL method shows a 13.8 % improvement in micro-average precision, and a 21.7 % improvement in micro-average recall, compared to the ImageJ method. Moreover, the DL method can process 70 images within 1 min, while the ImageJ method costs at least 6 h. The promising performance of our method allows it to offer a potential alternative to examine the interaction between microparticles and biomass in biological wastewater treatment process in an affordable manner. This approach offers more useful insights into the treatment process, enabling further reveal the microparticles transfer in biological treatment systems.