We investigated the effect of personality traits and flight experience on pilot cognitive and affective responses across seven startling and surprising scenarios performed in motion-based simulators. A dataset of 89 airline pilots from four studies was used. The personality traits measured were trait anxiety, decision-related action orientation (AOD), and failure-related action orientation (AOF). Pilot self-reported responses in scenarios were standardized by obtaining z scores of startle, surprise, stress, and mental workload. Only trait anxiety was found to be significantly positively correlated with stress. No significant effects of AOD, AOF, or flight hours were found on pilots’ responses. The results indicate trait anxiety may affect pilots’ responses to stressful scenarios, even though pilots are selected based on low trait anxiety.@en