Tyre characteristics can strongly affect bicycle dynamics, therefore the overall bicycle performances. However, it may be hard to measure lateral characteristics with low uncertainty. Proper test-rigs are needed to obtain reliable tyre parameters, to be used then for modelling. The paper presents VeTyT, acronym of “Velo Tyre Testing”, a new test-rig specifically developed for bicycle tyres at the Department of Mechanical Engineering of Politecnico di Milano. It is the first test-rig for bicycle tyres in compliance with the standard ISO 9001-2015. We also present the results of an experimental campaign conducted on a road racing bicycle tyre. In particular, the impact of rim stiffness is relevant to tyre characteristics, leading to a 13% increase in cornering stiffness under the same test conditions.