BACKGROUND: Working in an office environment is characterised by physical inactivity and sedentary behaviour. This behaviour contributes to several health risks in the long run. Dynamic workstations which allow people to combine desk activities with physical activity, may contribute to prevention of these health risks. OBJECTIVE: A dynamic workstation, called Oxidesk, was evaluated to determine the possible contribution to healthy behaviour and the impact on perceived work performance. METHODS: A field test was conducted with 22 office workers, employed at a health insurance company in the Netherlands. RESULTS: The Oxidesk was well accepted, positively perceived for fitness and the participants maintained their work performance. Physical activity was lower than the activity level required in the Dutch guidelines for sufficient physical activity. CONCLUSIONS: Although there was a slight increase in physical activity, the Oxidesk may be helpful in the reducing health risks involved and seems applicable for introduction to office environments.