Challenges and potential are embedded in peri-urban agriculture under metropolitan sprawl, which requires a future-oriented development to address major trends such as the climate crisis, metropolitan sprawl, autonomy in food production and environmental quality issues. Following a design exploration in Oosterwold, Almere, The Netherlands, a biophilic design framework was used to demonstrate the effective transformation of a symbiotic peri-urban agricultural interface. The results embody a sequence of principles based upon biophilic design, urban metabolism, and bottom-up governance mechanism.