Tel Tsaf is a Middle Chalcolithic (ca. 5200–4700 cal BC) site located in the central Jordan Valley, Israel. The site reflects increasing long-distance trade, organized cereal crop cultivation and possibly olive horticulture. Organic residue analysis of lipids recovered from 100 pottery vessels and three stone vessels and comparison to the botanical and faunal remains suggest that the vessels were used to contain or process various combinations of domestic animal and plant products and that these food products were supplemented by local wild plants. This shows direct continuity of dietary traditions from the preceding Neolithic period; however, there were some developments within the suite of domesticated resources, including the earliest direct evidence for milk exploitation in the southern Levant. Culinary traditions incorporated recipes where these food products were cooked and consumed together in various combinations. These dietary patterns were a part of daily life at Tel Tsaf, adding another layer to our understanding of the village and the culinary traditions of the Middle Chalcolithic period in the southern Levant.

Beehive products have a rich global history. In the wider Levantine region, bees had a significant role in Egypt and Mesopotamia, and intensive beekeeping was noted in Israel during the Biblical period when apiaries were first identified. This study investigates the origins of this extensive beekeeping through organic residue analysis of pottery from prehistoric sites in the southern Levant. The results suggest that beehive products from likely wild bees were used during the Chalcolithic period as a vessel surface treatment and/or as part of the diet. These functions are reinforced by comparison to the wider archaeological record. While the true frequency of beeswax use may be debated, alternatives to beehive products were seemingly preferred as wild resources contrasted with the socio-economic system centred on domesticated resources, controlled production and standardization. Bee products only became an important part of the economic canon in the southern Levant several millennia later.