Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Journal article (2017)

Authors

Bernadeth F. Ticar Ticar Iloilo Science and Technology University
Zuliyati Rohmah Universitas Gadjah Mada
Solange Mussatto Technical University of Denmark (DTU)
Jae Min Lim Changwon National University
Seongha Park Park Changwon National University
Byeong Dae Choi Choi Gyeongsang National University
Research Group
BT/Bioprocess Engineering (AS) (TU Delft)
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Published Date

01-04-2017

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Bioprocess Engineering

Abstract

Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3 > F2 > F1 > Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.

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