Ozonation and UV254 nm radiation for the removal of microorganisms and antibiotic resistance genes from urban wastewater

Journal article (2017)

Authors

José M. Sousa Universidade do Porto
Gonçalo Macedo Universidade Católica Portuguesa
Marta Pedrosa Universidade do Porto
Cristina Becerra-Castro Universidade Católica Portuguesa, Universidade do Porto
Sérgio Castro-Silva Centro Empresarial e Tecnológico
M. Fernando R. Pereira Universidade do Porto
Adrian M.T. Silva Universidade do Porto
Olga C. Nunes Universidade do Porto
Célia M. Manaia Universidade Católica Portuguesa
Affiliation
External organisation
More Info
expand_more

Published Date

2017

Language

English

Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Affiliation

External organisation

Abstract

Conventional wastewater treatment has a limited capacity to reduce antibiotic resistant bacteria and genes (ARB&ARG). Tertiary treatment processes are promising solutions, although the transitory inactivation of bacteria may select ARB&ARG. This study aimed at assessing the potential of ozonation and UV254 nm radiation to inactivate cultivable fungal and bacterial populations, and the selected genes 16S rRNA (common to all bacteria), intI1 (common in Gram-negative bacteria) and the ARG vanA, blaTEM, sul1 and qnrS. The abundance of the different microbiological parameters per volume of wastewater was reduced by ∼ 2 log units for cultivable fungi and 16S rRNA and intI1 genes, by ∼3–4 log units, for total heterotrophs, enterobacteria and enterococci, and to values close or below the limits of quantification for ARG, for both processes, after a contact time of 30 min. Yet, most of the cultivable populations, the 16S rRNA and intI1 genes as well as the ARG, except qnrS after ozonation, reached pre-treatment levels after 3 days storage, suggesting a transitory rather than permanent microbial inactivation. Noticeably, normalization per 16S rRNA gene evidenced an increase of the ARG and intI1 prevalence, mainly after UV254 nm treatment. The results suggest that these tertiary treatments may be selecting for ARB&ARG populations.