Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Journal article (2017)

Authors

Andreas Tosstorff DECHEMA Research Institute
Cora Kroner DECHEMA Research Institute
Diederik J. Opperman University of the Free State
F. Hollmann BT/Biocatalysis - AS
Dirk Holtmann DECHEMA Research Institute
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2017 Andreas Tosstorff, Cora Kroner, Diederik J. Opperman, F. Hollmann, Dirk Holtmann
DOI: https://doi.org/10.1002/elsc.201600158
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Published Date

2017

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.

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