A Photoenzymatic NADH Regeneration System

Journal article (2018)

Authors

G.T. Höfler BT/Biocatalysis - AS
E. Fernandez Fueyo BN/Greg Bokinsky Lab - AS
M. Pesic BT/Biocatalysis - AS
S.H.H. Younes BT/Biocatalysis - AS
Eun Gyu Choi Ulsan National Institute of Science and Technology
Yong H. Kim Ulsan National Institute of Science and Technology
Vlada B. Urlacher Universität Düsseldorf
I.W.C.E. Arends BT/Biotechnologie
F. Hollmann BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2018 G.T. Höfler, E. Fernandez Fueyo, M. Pesic, S.H.H. Younes, Eun Gyu Choi, Yong H. Kim, Vlada B. Urlacher, I.W.C.E. Arends, F. Hollmann
DOI: https://doi.org/10.1002/cbic.201800530
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Published Date

2018

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.