3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

Conference paper (2024)

Authors

J. de Wit ImPhys/Computational Imaging - AS , ImPhys/Kalkman group - AS
Sebastian Tonn Universiteit Utrecht
Mon-Ray Shao Universiteit Utrecht
Guido Van den Ackerveken Universiteit Utrecht
J. Kalkman ImPhys/Kalkman group - AS , ImPhys/Computational Imaging - AS
Research Group
ImPhys/Computational Imaging (AS) (TU Delft)
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Published Date

2024

Language

English

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Faculty

Applied Sciences

Department

ImPhys/Imaging Physics

Research Group

ImPhys/Computational Imaging

Abstract

Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.

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File under embargo until 04-03-2025