Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Journal article (2022)

Authors

S. Hung Team Carlas Smith - Mechanical, Maritime and Materials Engineering
Arnau Llobet Rosell University of Lausanne
Daphne Jurriens Universiteit Utrecht
O.A. Soloviev Team Michel Verhaegen - Mechanical, Maritime and Materials Engineering
Lukas C. Kapitein Universiteit Utrecht
K.S. Grußmayer Kavli institute of nanoscience Delft, BN/Kristin Grussmayer Lab - AS
Lukas J. Neukomm University of Lausanne
M.H.G. Verhaegen Team Michel Verhaegen - Mechanical, Maritime and Materials Engineering
C.S. Smith ImPhys/Computational Imaging - AS , Team Carlas Smith - Mechanical, Maritime and Materials Engineering
Research Group
Team Carlas Smith (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2022 S. Hung, Arnau Llobet Rosell, Daphne Jurriens, O.A. Soloviev, Lukas C. Kapitein, K.S. Grußmayer, Lukas J. Neukomm, M.H.G. Verhaegen, C.S. Smith
DOI: https://doi.org/10.3389/fnins.2022.954949
More Info
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Published Date

2022

Language

English

Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Carlas Smith

Abstract

Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.