Unveiling the limits of precision in iterative MINFLUX

Journal article (2024)

Authors

C.S. Smith Team Carlas Smith - Mechanical, Maritime and Materials Engineering
D. Kalisvaart Team Carlas Smith - Mechanical, Maritime and Materials Engineering
Kirti Prakash Royal Marsden NHS Foundation Trust
Research Group
Team Carlas Smith (Mechanical, Maritime and Materials Engineering) (TU Delft)
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Published Date

2024

Language

English

Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Carlas Smith

Abstract

In single-molecule microscopy, a big question is how precisely we can estimate the location of a single molecule. Our research shows that by using iterative localisation microscopy and factoring in the prior information, we can boost precision and reduce the number of photons needed. Leveraging the Van Trees inequality aids in determining the optimal precision achievable. Our approach holds promise for wider application in discerning the optimal precision across diverse imaging scenarios, encompassing various illumination strategies, point spread functions and overarching control methodologies.