Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

van Aalst, A.C.A.

doi:10.4233/9a149309-2339-40c5-9151-032d70b91dbe

Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

Doctoral thesis (2023)

Authors

A.C.A. van Aalst BT/Industriele Microbiologie -

Research Group

BT/Industriele Microbiologie () (TU Delft)

DOI: https://doi.org/10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe

Saccharomyces cerevisiae Redox engineering Ethanol production Co-culture Metabolic engineering Anaerobic fermentation Electrons Industrial biotechnology

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Published Date

2023

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Industriele Microbiologie

Abstract

The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate.

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