Spatial analysis of transcript and protein levels in skeletal muscle

Journal article (2024)

Authors

Paola Pisterzi The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center
Clara Martinez Mir The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center
Ouafa Dahri Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
Isabel de Poorter Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
Sandra Batlles Parera Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
M. Dostanić Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
Massimo Mastrangeli Electronic Components, Technology and Materials - EEMCS
Christine Mummery Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
Niels Geijsen Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
Fanny Sage The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center
Research Group
Electronic Components, Technology and Materials (EEMCS) (TU Delft)
More Info
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Published Date

2024

Language

English

Faculty

Electrical Engineering, Mathematics and Computer Science

Department

Microelectronics

Research Group

Electronic Components, Technology and Materials

Abstract

Skeletal muscle spatial analyses have revealed unexpected regionalized gene expression patterns challenging the understanding of muscle as a homogeneous tissue. Here, we present a protocol for the spatial analysis of transcript and protein levels in murine skeletal muscle. We describe steps for tibialis anterior dissection, formaldehyde fixation, tissue chopper cutting, and hybridization chain reaction (HCR) detection and amplification. We then detail procedures for immunostaining, tissue clearing, and imaging. This protocol is easily adaptable to other tissues.