An alginate-confined peroxygenase-CLEA for styrene epoxidation

Journal article (2021)

Authors

Y. Wu BT/Biocatalysis - AS
Matteo Planchestainer ETH Zürich
Martin Held ETH Zürich
Miguel Alcalde Institute of Catalysis and Petrochemistry, Madrid
F. Hollmann BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2021 Friederike E.H. Nintzel, Y. Wu, Matteo Planchestainer, Martin Held, Miguel Alcalde, F. Hollmann
DOI: https://doi.org/10.1039/d1cc01868j
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Published Date

2021

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

Oxyfunctionalisation reactions in neat substrate still pose a challenge for biocatalysis. Here, we report an alginate-confined peroxygenase-CLEA to catalyse the enantioselective epoxidation of cis-β-methylstyrene in a solvent-free reaction system achieving turnover numbers of 96 000 for the biocatalyst and epoxide concentrations of 48 mM.