Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Costantini, Irene; Menzel, Miriam; Silvestri, Ludovico; Schubert, Nicole; Axer, Markus; Amunts, Katrin; Pavone, Francesco Saverio

Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Conference paper (2017)

Authors

Irene Costantini University of Florence

Miriam Menzel Forschungszentrum Jülich

Ludovico Silvestri University of Florence

Nicole Schubert Forschungszentrum Jülich

Markus Axer Forschungszentrum Jülich

Katrin Amunts Universität Düsseldorf, Forschungszentrum Jülich

Francesco Saverio Pavone CNR-INO, University of Florence

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Published Date

2017

Language

English

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Abstract

In this work, we employ an integrated label-free dual approach that combines Polarized Light Imaging with Two-Photon Fluorescence Microscopy to study fiber orientations of myelinated axons in fixed brain slices from different species.