Biocatalytic synthesis of the Green Note trans-2-hexenal in a continuous-flow microreactor

Journal article (2018)

Authors

M.M.C.H. van Schie BT/Biocatalysis - AS
T. Pedroso de Almeida BT/Biocatalysis - AS
Gabriele Laudadio Eindhoven University of Technology
F. Tieves BT/Biocatalysis - AS
E. Fernandez Fueyo CSIC - Centro de Investigaciones Biologicas (CIB)
Timothy Noël Eindhoven University of Technology
I.W.C.E. Arends BT/Biotechnologie
F. Hollmann BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2018 M.M.C.H. van Schie, T. Pedroso de Almeida, Gabriele Laudadio, F. Tieves, E. Fernandez Fueyo, Timothy Noël, I.W.C.E. Arends, F. Hollmann
DOI: https://doi.org/10.3762/bjoc.14.58
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Published Date

26-03-2018

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

The biocatalytic preparation of trans-hex-2-enal from trans-hex-2-enol using a novel aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. As O2-dependent enzyme PeAAOx-dependent reactions are generally plagued by the poor solubility of O2 in aqueous media and mass transfer limitations resulting in poor reaction rates. These limitations were efficiently overcome by conducting the reaction in a flow-reactor setup reaching unpreceded catalytic activities for the enzyme in terms of turnover frequency (up to 38 s-1) and turnover numbers (more than 300000) pointing towards preparative usefulness of the proposed reaction scheme.

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