Characterization of the Old Yellow Enzyme Homolog from Bacillus subtilis (YqjM)

Journal article (2017)

Authors

M. Pesic BT/Biocatalysis - AS
E. Fernandez Fueyo BT/Biocatalysis - AS
F. Hollmann BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2017 M. Pesic, E. Fernandez Fueyo, F. Hollmann
DOI: https://doi.org/10.1002/slct.201700724
More Info
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Published Date

2017

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

Old Yellow Enzymes (OYEs) have become practical catalysts for the stereoselective trans-hydrogenation of conjugated C=C-double bonds. Despite their importance, in-depth characterization of their kinetic parameters and factors influencing their activity and stability have largely been neglected in the past years. In this study, we close this gap for one of the most prominent OYEs, i. e. the OYE from Bacillus subtilis (YqjM).